| 蛋黄中低密度脂蛋白对冻融后猪精子DNA完整性及受精率的影响 |
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| 引用本文： | 徐妲,金一,方南洙,韩明铭.蛋黄中低密度脂蛋白对冻融后猪精子DNA完整性及受精率的影响[J].中国畜牧杂志,2008,44(23). |
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| 作者姓名： | 徐妲 金一 方南洙 韩明铭 |
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| 作者单位： | 延边大学农学院,吉林,龙井,133400 |
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| 摘 要： | 本实验目的是评价低密度脂蛋白(LDL)对冻融后猪精子DNA完整性及受精率的影响。结果表明:添加9%LDL处理组冷冻解冻后,精细胞DNA完整率(彗星长为13.21,尾长为10.28,尾部DNA(%)为16.54,尾矩为2.31)和卵裂率(43.12%)都与7%、8%和10%LDL处理组差异显著(P<0.05)。本实验表明LDL能显著提高冷冻解冻后猪精子DNA的完整性;也能显著改善冷冻解冻后猪精子体外受精(IVF)率。
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| 关 键 词： | 精子 低密度脂蛋白 DNA IVF 猪 |
Effects of LDL on DNA Integrity and Fertilization Rate of Porcine Sperm after Freezing Thawing |
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| XU Da,JIN Yi,FANG Nan-zhu,HAN Ming-ming.Effects of LDL on DNA Integrity and Fertilization Rate of Porcine Sperm after Freezing Thawing[J].Chinese Journal of Animal Science,2008,44(23). |
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| Authors: | XU Da JIN Yi FANG Nan-zhu HAN Ming-ming |
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| Abstract: | The purpose of this study was to evaluate the effect of LDL (low density lipoprotein) on DNA integrity and fertilization rate of frozen-thawed porcine spermatozoa. The results showed that adding 9% LDLcan significantly increase DNA integrity (comet length,13.21;tail length,10.28; tail DNA(%),16.54;tail moment,2.31) and IVF rate(43.12%)of frozen-thawed sperm(P < 0.05). The experimental results showed adding LDL can improve DNA integrity and IVF rate of frozen-thawed sperm, but it will be further studied on their mechanisms. |
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| Keywords: | DNA IVF |
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