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| 水稻抗白叶枯病近等基因系CBB30的培育及Xa30(t)的初步定位 | |
| 引用本文： | 金旭炜,王春连,杨清,江祺祥,樊颖伦,刘古春,赵开军.水稻抗白叶枯病近等基因系CBB30的培育及Xa30(t)的初步定位[J].中国农业科学,2007,40(6):1094-1100. |
| 作者姓名： | 金旭炜 王春连 杨清 江祺祥 樊颖伦 刘古春 赵开军 |
| 作者单位： | 1. 南京农业大学生命科学学院,南京,210095;中国农业科学院作物科学研究所/农业部作物遗传育种重点实验室,北京,100081 2. 中国农业科学院作物科学研究所/农业部作物遗传育种重点实验室,北京,100081;国家农作物基因资源与基因改良重大科学工程,北京,100081 3. 南京农业大学生命科学学院,南京,210095 4. 江苏省常州市武进区稻麦育种场,常州,213149 |
| 摘 要： | 【目的】将普通野生稻资源Y238所携有的抗白叶枯病新基因Xa30(t)导入栽培稻JG30，培育近等基因系，进行分子标记定位，以便应用于育种实践。【方法】以感病籼稻品种JG30为轮回亲本，Y238为供体进行杂交、回交、自交培育近等基因系；以JG30/Y238的一个BC6F2代群体为定位群体，利用分离集团分析法（BSA），借助SSR、EST、STS等分子标记对Xa30(t)进行分子标记定位。【结果】成功培育了携有Xa30(t)基因的水稻抗白叶枯病近等基因系CBB30。从343个分子标记中筛选出4个能揭示抗感多态性的标记RM1341、V88、C189及03STS，用该4个标记对BC6F2代群体303个单株进行分子检测和连锁分析，结果表明上述4个标记均位于水稻第11染色体长臂，与Xa30(t)的遗传距离分别为11.4 cM、11.4 cM、4.4 cM及2.0 cM，且它们位于Xa30(t)基因的同一侧。【结论】通过构建近等基因系及分子标记检测找到4个与Xa30(t)基因连锁的标记RM1341、V88、C189及03STS，将Xa30(t)基因定位于水稻第11染色体长臂上。 |
| 关 键 词： | 水稻白叶枯病 Xa30(t) 近等基因系 分子标记定位 |
| 收稿时间： | 2006-4-17 |
| 修稿时间： | 2006年4月17日 |
Breeding of Near-Isogenic Line CBB30 and Molecular Mapping of Xa30(t), a New Resistance Gene to Bacterial Blight in Rice |
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| JIN Xu-wei,WANG Chun-lian,YANG Qing,JIANG Qi-xiang,FAN Ying-lun,LIU Gu-chun,ZHAO Kai-jun.Breeding of Near-Isogenic Line CBB30 and Molecular Mapping of Xa30(t), a New Resistance Gene to Bacterial Blight in Rice[J].Scientia Agricultura Sinica,2007,40(6):1094-1100. | |
| Authors: | JIN Xu-wei WANG Chun-lian YANG Qing JIANG Qi-xiang FAN Ying-lun LIU Gu-chun ZHAO Kai-jun |
| Abstract: | Abstract： Bacterial blight (BB) caused by Xanthomonas oryzae pv oryzae (Xoo) is one of the most serious diseases in rice. Host resistance is thought to be a sound approach to control BB. A new bacterial blight resistance germplasm (Y238) was identified from the wild rice species Oryza rufipogon and the resistance locus was transferred to the susceptible Indica rice variety JG30 by cross, backcross and self-cross to BC6F3 generation. The resistance locus was tentatively designated as Xa30（t）. A BC6F2 population was used for molecular mapping of Xa30（t）. Inoculation test of the BC6F2 population at adult stage with the Philippine strain PXO99 of Xoo showed that the segregation ratio between resistant and susceptible plants was 3:1, indicating that a dominant locus controls the resistance. Bulked Segregant Analysis (BSA) was adopted to survey 343 PCR-based SSR, EST and STS molecular markers located on the twelve rice chromosomes and results showed that four markers, RM1341, V88, C189 and 03STS, displayed polymorphism between the susceptible-pool and resistance-pool. By analyzing the 303 individuals of the BC6F2 population with the mentioned four markers together with their resistance phenotypes, the gene Xa30（t）was mapped on the long arm of chromosome 11. Linkage analysis revealed that RM1341, V88, C189 and 03STS located on the same side of Xa30（t）, with genetic distances of 11.4cM, 11.4cM , 4.4 cM and 2.0 cM respectively. |
| Keywords: | Xa30(t) |
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