Expression Analysis of C-type Natriuretic Peptide and Its Receptor in Buffalo Follicles |
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| Authors: | ZHOU Wen-ting SUN Long-fei YIN Na DU Shan-shan ZHAO Xin DAI Xiao-li LU Feng-hua SHI De-shun |
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| Institution: | State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, China |
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| Abstract: | In order to investigate the expression pattern of C-type natriuretic peptide（CNP）and its receptors（NPR）in buffalo follicles,firstly,the mRNA expression level of ANP,BNP,CNP,NPR1 and NPR2 in ovary were assayed by Real-time quantitative PCR;Secondly,the protein expression of CNP and NPR2 in buffalo follicles were detected by immunohistochemical stainning method;Lastly,the mRNA expression level of CNP and NPR2 in granule cells and cumulus cells were assayed by Real-time quantitative PCR.The results showed that CNP gene and its receptor NPR2 mainly expressed in buffalo ovary,the protein of CNP and NPR2 expressed in all stages of follicles,CNP mainly expressed in mural granulosa cells and NPR2 primarily presented in cumulus cells,the mRNA expression of CNP gene in granule cells was significantly higher than cumulus cells（P<0.05),whereas the mRNA expression of NPR2 gene in cumulus cells was significantly higher than granule cells（P<0.05).In conclusion,among the main members of natriuretic peptides and its receptors,CNP and NPR2 presented strong expression in buffalo ovary.CNP mainly expressed in mural granulosa cells,but NPR2 primarily presented in cumulus cells which arounding oocytes. |
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| Keywords: | C-type natriuretic peptide natriuretic peptide receptor 2 expression analysis buffalo follicles |
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