Expression of MPB70 Protein and Identification the Immunogenicity of Deer Mycobacterium tuberculosis |
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| Authors: | WANG Xiao-long LU Tian-cheng WANG Xiu-ran YANG Yan-ling |
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| Institution: | 1. College of Life Science, Jilin Agricultural University, Changchun 130118, China;
2. Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China |
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| Abstract: | In this study,the immunogenicity protein MPB70 gene was amplified from Mycobacterium tuberculosis genome DNA which separated from deer, and about 590 bp fragment was obtained. Then the fragment was cloned and constructed prokaryotic expression vector of pET-30a-MPB70, and the recombinant plasmid was put into E. coli BL21(DE3).Purified after IPTG induction, and analyzed by SDS-PAGE, a specificity protein band was observed at 20 ku. Using the deer serum positive of tuberculosis in Western blotting, the fusion protein could be combined with deer serum positive of tuberculosis antibody and arise specific immune response. The protein could be used as a specific antigen to test the deer tuberculosis. The study laid a foundation for further studying the deer tuberculosis appraisal method. |
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| Keywords: | Mycobacterium tuberculosis MPB70 gene prokaryotic expression |
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