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| 酵母诱导表达鸡IFN-α抗IBDV效果及其对信号分子PI3K和NF-κB的影响 | |
| 引用本文： | 韩海锋,李银聚,程相朝,张春杰,余祖华,杨惠,杨荣恩,付威.酵母诱导表达鸡IFN-α抗IBDV效果及其对信号分子PI3K和NF-κB的影响[J].中国畜牧兽医,2016,43(8):2164-2169. |
| 作者姓名： | 韩海锋 李银聚 程相朝 张春杰 余祖华 杨惠 杨荣恩 付威 |
| 作者单位： | 1. 河南科技大学动物疫病与公共安全重点实验室, 洛阳 471003; 2. 河南天纳图生物科技有限公司, 漯河 462600 |
| 基金项目： | 河南省科技攻关项目（132102110117） |
| 摘 要： | 试验旨在研究酵母表达鸡IFN-α抗传染性法氏囊病病毒（IBDV）的效果及对其淋巴细胞信号分子PI3K和NF-κB p65的影响。将40只10日龄非免疫雏鸡随机分为IFN-α组和空白对照组，持续注射给药3 d后，于第3次给药后第1天开始每天心脏采血，持续3 d，并于最后一次采血后对雏鸡进行攻毒，在攻毒后开始每天心脏采血，连续3 d。通过MTT法检测淋巴增殖活性情况，ELISA测定不同时间段淋巴细胞中PI3K的水平、细胞中总NF-κB p65、细胞核中NF-κB p65蛋白表达含量及IBDV抗原量。结果显示，与对照组相比，攻毒前，IFN-α对淋巴增殖活性、细胞中PI3K和NF-κB p65的表达具有极显著地促进作用（P<0.01）；与攻毒前相比，攻毒后第1和2天IFN-α组淋巴增殖活性、细胞中PI3K和NF-κB p65的表达极显著增加（P<0.01），IFN-α对IBDV-Ag具有极显著地抑制效果（P<0.01）。这些结果表明，IFN-α可通过增加PI3K激活NF-κB信号通路以增强机体免疫反应，并对IBDV-Ag有显著的抑制作用，本试验为IFN-α免疫增强和抗病毒作用的研究提供科学依据。 |
| 关 键 词： | IFN-&alpha PI3K 核转录因子 NF-&kappa B p65 传染性法氏囊病 |
| 收稿时间： | 2015-12-28 |
Study on the Anti-IBDV Activity of Chicken IFN-α and Its Effects on Signaling Molecule PI3K and NF-κB |
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| HAN Hai-feng,LI Yin-ju,CHENG Xiang-chao,ZHANG Chun-jie,YU Zu-hua,YANG Hui,YANG Rong-en,FU Wei.Study on the Anti-IBDV Activity of Chicken IFN-α and Its Effects on Signaling Molecule PI3K and NF-κB[J].China Animal Husbandry & Veterinary Medicine,2016,43(8):2164-2169. | |
| Authors: | HAN Hai-feng LI Yin-ju CHENG Xiang-chao ZHANG Chun-jie YU Zu-hua YANG Hui YANG Rong-en FU Wei |
| Institution: | 1. Key Laboratory of Animal Disease and Public Security, Henan University of Science and Technology, Luoyang 471003, China; 2. Henan Tian Na Tu Biotechnology Co., Ltd., Luohe 462600, China |
| Abstract: | To investigate the anti-IBDV activity of IFN-α and its effects on chicken lymphocyte signaling molecule PI3K and NF-κB activity,forty 10-day-old non-immune chickens were randomly separated into two groups:Blank control group and IFN-α group.The chicken of two groups were inoculated with IFN-α and saline via intramscular injection for three days,respectively.Then they were inoculated with IBDV.From 13 to 18-day-old,the lymphocytes were isolated from peripheral blood of chicken.The lymphocyte proliferation of chicken were detected by the MTT method,the PI3K and NF-κB p65 concentration in lymphocyte and the IBDV-Ag of serum were detected at different time with ELISA.The results showed that compared with the blank control group,IFN-α could stimulate chicken lymphocyte proliferation and the PI3K and NF-κB p65 concentration in chicken lymphocyte were extremely significantly increased before infection (P<0.01).The lymphocyte proliferation and the expression of PI3K and NF-κB p65 in cell after IBDV infection were extremely significantly higher than before (P<0.01).IFN-α could extremely significantly inhabit the proliferation of IBDV-Ag (P<0.01).IFN-α could significantly promote lymphocyte transformation by increasing PI3K concentration and the biological activity of lymphocyte transformation associated with the PI3K and NF-κB p65 expression,activation and nucleo-cytoplasmic transport.It also could reduce IBDV-Ag proliferation in chickens.The study built foundation for IFN-α protein immuno enhancement and anti-viral activity. |
| Keywords: | IFN-α PI3K nuclear factor NF-κB p65 IBD |
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