Polymorphism of SNP Site rs80894395 and its Association with the Body Size of Xiang Pig |
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| Authors: | WANG Yang MEI Zi-ling YU Chang-yan NIU Xi XIE Ling-ling NIU Qiu-jin QIU Zhi-lang WANG Jia-fu RAN Xue-qin |
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| Institution: | 1. College of Life Sciences, Guizhou University, Guiyang 550025, China;2. Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China;3. College of Animal Science, Guizhou University, Guiyang 550025, China |
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| Abstract: | To explore the functional genes affecting the growth and development of Xiang pig, a single nucleotide polymorphism (SNP) site rs80894395, located at intron 10 of polypeptide N-acetylgalactosaminyltransferase 2 (GALNT2) gene was screened out by Illumina Porcine SNP60K chip, which showed a weak association with the abdomen circumference of pig.Further, the genotypes of site rs80894395 in 520 female Xiang pigs were detected using allele-specific PCR(AS-PCR) method, taking 43 Yorkshire pigs and 36 Kele pigs as control groups.All of CC, CT and TT genotypes were detected from the three pig breeds.The dominant genotype of Xiang pig was CT genotype and it was positively weak-associated with its body measurement indexes including body weight, body length, and abdomen circumference.The CC genotype was dominant genotype in Yorkshire pig, and the frequency of T allele was extremely significant decreased compared with that of Xiang pig (P<0.01).There was no difference in the frequencies of CC and CT genotypes in Kele pig, and its allele frequencies was not significantly different compared with that of Xiang or Yorkshire pig breeds (P>0.05).A splice-site nearby site rs80894395 was found out using bioinformatics prediction software, which might affect the splicing process of GALNT2 gene.It suggested that site rs80894395 might have a connection with the growth and development of Xiang pig. |
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| Keywords: | Xiang pig body sizes intron splicing SNP GALNT2 gene |
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