Effects of Different Adjuvants on the Immunogenicity of PCV2-Cap Protein |
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| Authors: | WANG Li-bo WU Run-sheng YAN Chao LV Tun |
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| Institution: | 1. College of Biological Science and Engineering, Fuzhou University, Fuzhou 350116, China;
2. FuZhou Da Bei Nong Biotech Co., Ltd., Fuzhou 350014, China |
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| Abstract: | The study was aimed to prepare vaccines with different adjuvants,and research its effects on immunogenicity.The PCV2 Cap gene with its signal peptide removed was connected to pET-28a vector,and then was induced to express,using sodium deoxycholate（DOC）and low concentration of urea to dissolve the inclusion body.Different adjuvants,such as alum-based adjuvants,liposome adjuvants,propolis adjuvants,white oil adjuvants and Freund adjuvant were prepared,together with protein purified to make up subunit vaccines,and commercial inactivated vaccine as positive control,immuning mice,and ELISA method were used to detect changes in concentrations of animal serum antibody and cytokines,evaluated the immune protective effect.Results showed that the expression product in the form of inclusion body,using the DOC could omit dissolving inclusion body protein renaturation steps,and the purification method was simple,the capsid protein obtained had high purity.ELISA assays showed that PCV2-Cap had good immunogenicity.And we found that the water system adjuvants had high immune activity,this could provide important previous experimental data for the commercialization of the subunit vaccine. |
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| Keywords: | porcine circovirus inclusion body adjuvant subunit vaccine |
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