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| 贵州省猪源沙门氏菌对β-内酰胺类药耐药性及耐药基因分析 | |
| 引用本文： | 曹正花,谭艾娟,吕世明,王雄,杜国琴.贵州省猪源沙门氏菌对β-内酰胺类药耐药性及耐药基因分析[J].中国畜牧兽医,2016,43(7):1737-1742. |
| 作者姓名： | 曹正花 谭艾娟 吕世明 王雄 杜国琴 |
| 作者单位： | 1. 贵州大学生命科学学院, 贵阳 550025; 2. 贵州大学动物科学学院, 贵阳 550025 |
| 基金项目： | 贵州省科技厅农业攻关计划项目"生鲜乳安全保障关键技术集成与示范"（黔科合NZ字[2012]3011号） |
| 摘 要： | 为了解贵州省猪源沙门氏菌对β-内酰胺类抗菌药物耐药性及其耐药基因的流行情况，本试验从贵州省9个地区规模养猪场中分离鉴定130株沙门氏菌，采用微量肉汤稀释法测定其对常用的8种β-内酰胺类抗菌药物的敏感性，并用PCR法对β-内酰胺酶耐药基因进行检测。结果显示，沙门氏菌对常用的β-内酰胺类抗菌药物耐药性十分严重，其中对头孢他啶的耐药率为100%，其次是氨苄西林和阿莫西林，耐药率分别为80.77%和76.15%，耐药率最低的是头孢噻呋和头孢氨苄，均为46.15%。所有菌株均为多重耐药，其中最少为二重，占总数的2.31%，最多为八重，占总数的4.62%，多重耐药主要集中在四至七重，占总数的88.46%。PCR结果显示，SHV耐药基因未检出，TEM、OXA、CTX-M 3种基因检出率分别是85%、75%和46%，细菌的耐药性与相关耐药基因的检出率基本呈正相关。结果表明，猪源沙门氏菌对β-内酰胺类药物具有普遍耐药性，其中头孢他啶尤为严重。TEM、OXA、CTX-M基因是贵州省猪源沙门氏菌主要耐药基因，临床日益严重的耐药现象与耐药基因的普遍存在有很大的关系。 |
| 关 键 词： | 沙门氏菌 &beta -内酰胺药物 药敏试验 耐药基因 耐药表型 |
| 收稿时间： | 2015-11-25 |
Analysis of Drug Resistance and Resistant Genes of Salmonella to β-lactams Antimicrobial Agents Isolated from Pigs in Guizhou Province |
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| CAO Zheng-hua,TAN Ai-juan,LV Shi-ming,WANG Xiong,DU Guo-qin.Analysis of Drug Resistance and Resistant Genes of Salmonella to β-lactams Antimicrobial Agents Isolated from Pigs in Guizhou Province[J].China Animal Husbandry & Veterinary Medicine,2016,43(7):1737-1742. | |
| Authors: | CAO Zheng-hua TAN Ai-juan LV Shi-ming WANG Xiong DU Guo-qin |
| Institution: | 1. College of Life Science, Guizhou University, Guizhou 550025, China; 2. College of Animal Science, Guizhou University, Guizhou 550025, China |
| Abstract: | In order to analyse the resistance to β-lactams antimicrobial agents and the prevalence of resistant genes of Salmonella in Guizhou province,130 Salmonella strains were isolated and identified from 9 different regions of scale pig farms.The drug sensitivity to 8 kinds of β-lactams antimicrobial agents were determined by using the broth microdilution method.All β-lactams resistant isolates were detected for the presences of TEM,OXA,CTX-M and SHV genes by PCR.The results showed that drug resistance of Salmonella to the commonly used β-lactams antimicrobial agents was very serious,and the resistance rate to ceftazidime was the highest (100%),followed by ampicillin and amoxicillin,were 76.15% and 80.77%,respectively.The resistance rates of ceftiofur and cephalexin were the lowest (46.15%).Salmonella strains were all of multiple drug resistance,of which double resistance was at lowest (2.31%),and eightfold resistance was highest (4.62%),multidrug resistance mainly concentrated in fourfold to sevenfold,accounted for 88.46%.PCR results showed that TEM,OXA,CTX-M genes detection rate were 85%,75% and 46%,respectively,while the SHV gene was not inspected.Resistant phenotype was basically consistent with resistant genes.The results indicated that the resistance of Salmonella stains from pig to β-lactams antimicrobial agents were widespread,and ceftazidime was particularly serious.The TEM,OXA and CTX-M genes were mainly carried β-lactams resistant genes in Salmonella isolates from Guizhou province.It had a great relationship between the prevalence of resistance genes and growth of antimicrobial resistance. |
| Keywords: | Salmonella β-lactamas drugs drug sensitivity test resistance genes resistant phenotypes |
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