Prokaryotic Expression of Partial N Gene Fragment of Porcine Epidemic Diarrhea Virus and Reactionogenicity Analysis of the Expressed Protein |
| |
| Authors: | ZHANG Bo LI Shou-jun LV Mao-jie YANG Bao-shou |
| |
| Institution: | 1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China;2. Key Laboratory of Biogenic Veterinary Drugs Innovation, Ministry of Agriculture, Tianjin 300308, China;3. Ringpu Biological Research Institute, Tianjin 300308, China |
| |
| Abstract: | This experiment was aimed to study the antigenicity of prokaryotic expression of the N gene fragment of porcine epidemic diarrhea virus (PEDV),and lay a foundation for establishing an indirect ELISA method of PEDV.The N gene segment was amplified by RT-PCR,then the recombinant plasmid with the vector pET-30a(+) was constructed,which was induced by 0.5 mmol/L IPTG at 37 ℃ for 4 h.Furthermore,the expressed product was analyzed by SDS-PAGE and Western blotting.Sequencing results proved that recombinant plasmid was correctly constructed.The result showed that the PEDV polyclonal antibody could specifically bind to PEDV N protein,which indicated that the recombinant fusion protein had excellent immunogenicity.A prokaryotic expression vector for the fragment of N protein was successfully constructed in this study,which laid a foundation for the development of diagnosis of PEDV. |
| |
| Keywords: | porcine epidemic diarrhea virus (PEDV) N gene fragment prokaryotic expression reactionogenicity |
|
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
