Differential Expression and Function Prediction of miR-383 in Yak and Cattle-yak Testis |
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| Authors: | LIAO Ke CHAI Zhi-xin ZHANG Si-yuan ZHONG Jin-cheng |
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| Institution: | 1. Key Laboratory of Animal Genetics & Breeding, State Ethnic Affairs Commission and Ministry of Education, Southwest University for Nationalities, Chengdu 610041, China;2. Institute of Qinghai-tibetan Plateau, Southwest University for Nationalities, Chengdu 610041, China |
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| Abstract: | The aim of this study was to detect the differential expression of miR-383 in yak and cattle-yak testis,and explore the important roles of miR-383 in spermatogenesis.Using the testis tissue of mature yak and cattle-yak as experimental material,Real-time PCR was performed to detect the miR-383 expression.miR-383 targets were obtained using TargetScan and miRanda database,and biological functions of these target genes were predicted by David and Gene Ontology online softwares.The miR-383 had significantly differential expression in F1 testis tissue between yak and cattle-yak(P<0.05),and it had the highest expression level in yak testis tissues.131 target genes were obtained by target gene prediction which involved in cell proliferation,apoptosis,regulation of nucleic acid synthesis and other processes by GO analysis and KEGG pathway analysis.The results showed that miR-383 might participate in the regulation of germ cell differentiation and spermatogenesis,which would provide clues and theoretical basis for studying the function and regulation mechanism of miR-383 in cattle-yak germ cell. |
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| Keywords: | yak cattle-yak miR-383 spermatogenesis male sterility bioinformatics analysis |
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