Isolation,O Serogrouping and Drug Sensitivity Test of Avian Pathogenic Escherichia coli |
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| Authors: | MA Xing-shu ZHU Mei-xia SHI Hu YANG Guang-ping |
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| Institution: | 1. Laboratory of Molecular Biology, College of Agricultural, Hebei University of Engineering, Handan 056021, China;
2. Hexin Scientific and Technologic Center of Hebei Kexing Pharmaceutical Co., Ltd., Shijiazhuang 050200, China |
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| Abstract: | One hundred and ninety four avian pathogenic Escherichia.coli (APEC) isolated from different pathotypes of chickens in Hebei province,Shandong province,Henan province,Jiangsu province,Shanxi province and Liaoning province in China between May 2005 and March 2016 were investigated by determination of biochemical characters,O serogroup identification and drug sensitivity test.Out of 194 isolates, 124 strains(63.9%) could be grouped to serogroups O1(3),O2(26),O57(15),O65(47) and O78(33).The drug sensitivity test used eight antibiotics (ampicillin, neomycin, doxycycline, sulfamonomethoxine,tilmicosin,ciprofloxacin ceftiofur and florfenicol) by one strain of O1,O2,O57,O65,O78 serogroup,respectively.The results showed that all strains were sensitive to ciprofloxacin and neomycin, and resistance to other drugs at varying degrees.This study demonstrated that the high prevalence of O65,O78,O2 and O57 isolates existed and provided the basis for the development of multivalent bacterin in future. |
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| Keywords: | avian pathogenic E coli isolation identification O serogroups drug sensitivity test |
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