Mutation Breeding and Optimization of Enzyme-producing Condition of Bacillus lichenniformis Producing Cellulase |
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| Authors: | YU Zu-hua DING Ke HOU Kui LI Yuan-xiao LI Wang LIU Yi-chen CAO Ping-hua JIA Yan-yan CHENG Xiang-chao |
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| Institution: | 1. Key Laboratory of Animal Disease and Public Health of Henan University of Science and Technology, Luoyang 471003, China;2. Hongxiang Biological Feed Laboratory of Henan University of Science and Technology, Luoyang 471003, China |
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| Abstract: | In order to improve the ability of producing cellulase of Bacillus lichenniformis, the mutational strain was screened after Bacillus lichenniformis LY02 was induced by UV.Then the best optimal enzyme-producing condition, inoculum size, temperature, incubation time, initial pH and metal ions were studied.The results showed that the cellulase activity of mutated strain was increased by 34.31%.And the best optimal ferment conditions were as follows:Inoculation concentration was 1.5%, cultivated temperature was 37℃, ferment time was 24 h, initial pH of medium was 5.0, and K+ and Ba2+ could promote the cellulase producing.These results were contributed to develop the strain to be applied in practice. |
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| Keywords: | Bacillus licheniformis cellulase mutation enzyme-producing condition enzyme activity |
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