Effects of all-trans retinoic acid on sensitivity of gastric cancer cells to radiotherapy |
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| Authors: | WANG Yan-ping ZHAO Xian-qun ZHANG Xiang-dong XU Wei XIANG Xiao-hui |
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| Institution: | 1. Department of Gastroenterology, Nanyang Central Hospital, Nanyang 473000, China;
2. Center of Hepatobiliary, Pancreatic and Splenic Diseases, Affiliated Hospital of Logistical College, CPLA, Tianjin 300162, China |
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| Abstract: | AIM: To investigate the effect of all-trans retinoic acid (ATRA) on the viability of gastric cancer cell SGC-7901 and the sensitivity to radiotherapy. METHODS: MTT assay was used to examine the cell viability. Radio-sensitivity and cell cycle were determined by colony formation assay and flow cytometry, respectively. The mRNA levels of Bax, Bcl-2, survivin and NF-κB in the cells were measured by RT-qPCR. RESULTS: ATRA inhibited the viability of SGC-7901 cells in a concentration-dependent manner. The maximal inhibition was at concentration of 8 μmol/L. Colony formation assay revealed that the combination of ATRA with X-ray treatment significantly reduced the values of D0 and Dq, and shifted down the fitting survival curve, as compared with radiotherapy alone. Moreover, ATAR markedly decreased the percentage of G2/M phase in the SGC-7901 cells (P<0.05). In addition, following ATRA treatment, the mRNA levels of Bcl-2 and survivin were decreased (P<0.05), whereas the mRNA levels of Bax and NF-κB were increased (P<0.05). CONCLUSION: ATRA enhances the sensitivity of SGC-7901 cells to radiotherapy, inhibits G2/M arrest and regulates the mRNA expression of Bax, Bcl-2, survivin and NF-κB. |
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| Keywords: | All-trans retinoic acid Radiotherapy Gastric cancer cell SGC-7901 Sensitivity |
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