Effect of Canine Pyometra on IGF-2, IGF-1R Proteins Expressions of Endometria |
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| Authors: | DU Miao YUE Ying-wei ZHOU Peng-yang LI Shuang MA Wen-zhi |
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| Institution: | 1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China;2. SinoPharm (Tianjin) Medical EquiPment Co., Ltd., Tianjin 300022, China |
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| Abstract: | To investigate the expression of IGF-2 and IGF-1R protein in endometria of canine pyometra and analyze the possible correlation with the incidence of canine pyometra.Immunohistochemistry PV method and Western blotting were used to measure the IGF-2 and IGF-1R expression characteristic of 5 normal endometria and 19 endometria of canine pyometra.The results showed that the IGF-2 level of endometria from pyometra group was extremely significant higher than that of control group by immunohistochemistry and Western blotting (P<0.01).The positive expression rate of IGF-1R in endometria of canine pyometra compared with that in the normal endometria was extremely significant higher by immunohistochemistry and Western blotting (P<0.01).The correlation analysis of IGF-2 and IGF-1R in canine pyometra showed that the result of immunohistochemistry was low correlation (ｒ=0.423), the result of Western blotting was high correlation (ｒ=0.927).The data suggested that increased expression of IGF-2 and IGF-1R played an important role in occurrence of canine pyometra. |
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| Keywords: | IGF-2 IGF-1R canine pyometra immunohistochemistry Western blotting |
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