| 间歇浸没式生物反应器培养铁皮石斛组培苗 |
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| 引用本文： | 胡燕花,张本厚,贾明良,陈集双.间歇浸没式生物反应器培养铁皮石斛组培苗[J].中国农业科技导报,2016,18(3):190-194. |
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| 作者姓名： | 胡燕花 张本厚 贾明良 陈集双 |
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| 作者单位： | 1.南京工业大学大丰海洋产业研究院, 江苏 盐城 224100, 2.九江学院, 江西 九江 332005,3.南京工业大学, 南京 211800 |
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| 基金项目： | 江苏省自然科学基金项目（BK20141265）资助。 |
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| 摘 要： | 目前铁皮石斛种苗生产主要采用植物组织培养的方法,传统的固体培养方式成本高效率低,无法满足市场需求,利用间歇浸没式生物反应器可以减少人工投入,降低生产成本。利用自主研发的间歇浸没式生物反应器培养铁皮石斛种苗,同时以固体培养作对照,比较了两种培养方式的铁皮石斛增殖率及植株高度、茎粗、根系情况、移栽成活率等。结果显示间歇浸没式生物反应器培养获得的铁皮石斛增殖率更高,组培苗更健壮,而且根系适中,利于栽培,移栽成活率也高于固体培养,为铁皮石斛种苗生产提供了一种新的方法。
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| 关 键 词： | 植物组织培养 间歇浸没生物反应器 铁皮石斛 增殖率 |
Seedlings Cultivation of Dendrobium offiicinale using Temporary Immersion Bioreactors |
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| HU Yan-hua,ZHANG Ben-hou,JIA Ming-liang,CHEN Ji-shuang.Seedlings Cultivation of Dendrobium offiicinale using Temporary Immersion Bioreactors[J].Journal of Agricultural Science and Technology,2016,18(3):190-194. |
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| Authors: | HU Yan-hua ZHANG Ben-hou JIA Ming-liang CHEN Ji-shuang |
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| Institution: | 1.Dafeng Marine Industry Research Institute, Nanjing Technology University, Jiangsu Yancheng 224100| 2.Jiujiang University, Jiangxi Jiujiang 332005|3.Nanjing Tech University, Nanjing 211800, China |
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| Abstract: | At present, Dendrobium officinale seedlings are mainly produced by plant tissue culture. Solid culture method is characterized by high production costs and low multiplication rates, which is unable to satisfy the increasing market demands. Temporary immersion bioreactors（TIB）can reduce labor and costs of production. This study compared the multiplication rates, plant height, diameter of stem, root system growth and survival rates after transplantation of D. officinale plantlets cultured in self-developed TIB and solid medium. The multiplication rates and quality of plantlets produced in TIB were significantly higher than those produced in solid medium. Plantlets produced in TIB with moderate root size were suitable for cultivation, and their survival rates after transplantation were significantly higher than those produced in solid medium. These results showed that TIB provided a new protocol for production of D. officinale seedlings. |
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| Keywords: | plant tissue culture temporary immersion bioreactors Dendrobium officinale multiplication rates |
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