Diagnosis of Duck Hepatitis Virus Type 1 and Serotype 11 Riemerella anatipestifer Mixed Infection and Isolation and Identification of Pathogens |
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| Authors: | CHEN Cui-teng CHENG Long-fei FU Guang-hua WAN Chun-he LIU Rong-chang FU Qiu-ling CHEN Zhen ZHU Chun-hua CHEN Hong-mei SHI Shao-hua HUANG Yu |
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| Institution: | Fujian Key Laboratory of Poultry Disease Prevention and Control, Fujian Animal Disease Control Technology Development Center, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Science, Fuzhou 350013, China |
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| Abstract: | To determine the pathogens that caused the death of the 10-day-old sheldrakes of a outbroke epidemic duck farm at the end of June 2015,at Cangshan District in Fuzhou,the liver,spleen and brain of dead sheldrakes were collected,and then tested by virus isolation,isolation and purification of bacteria,PCR detection,serology and drug susceptibility test.The results showed duck hepatitis virus type 1 and 11 type Riemerella anatipestifer were detected and isolated,and the isolated duck hepatitis virus type 1 had the highest homology with MPZJ1206 strain and GD strain,and the percentage of homology were 98.7%.Laboratory isolation and identification of pathogen determined that the case was duck hepatitis virus type 1 and serotype 11 Riemerella anatipestifer mixed infection. |
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| Keywords: | DHAV-1 serotype 11 Riemerella anatipestifer isolation and identification |
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