Variation of CD8 after traumatic brain injury in serum of rats |
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| Authors: | LI Wei ZHENG Ran CAI Kang ZHENG Kuan WANG Zhao-rui |
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| Institution: | 1. Department of Neurosurgery, The First Affiliated Hospital, Jinan University, Guangzhou 510630, China;
2. The Center of Clinical Medical Research, Hongkong University, Hongkong, China |
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| Abstract: | AIM: To explore the character of CD8 after traumatic brain injury (TBI) in peripheral blood. METHODS: Improved Feeney's free-fall method was used to set up traumatic brain injury model. The levels of neuron-specific enolase (NSE) and CD8 in the serum of the rats were detected by ELISA. The correlation of both NSE and CD8 in the serum was analyzed by Pearson product-moment correlation. The FasL expression in the lymphocytes of peripheral blood was determined by Western blot. RESULTS: The elevated level of CD8 lagged behind the level of NSE in the serum after TBI. The serum level of NSE was significantly increased at the 1st day after TBI and reached a peak at the 3rd day, subsequently gradually decreased to a lower level; the serum level of CD8 was increased at the 3rd day after TBI, and reached a peak at the 7th day, then gradually decreased. The serum levels of NSE at 1st, 3rd and 7th days were positively correlated with the serum level of CD8 at 3rd, 7th and 14th days. However, the FasL expression in the CD8+ lymphocytes of peripheral blood showed no variation at different time points after TBI. CONCLUSION: NSE in the serum released from neural tissues after TBI stimulates immune response and induces the augmentation of CD8 in peripheral blood, which may be a cause of secondary injuries in the brain. |
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| Keywords: | Traumatic brain injury Neuron-specific enolase CD8 FasL |
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