Isolation and Identification of a Field Strain of Marek's Disease Virus and Analysis of Major Pathogenic Genes |
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| Authors: | LI Si-fei SUN Peng CHEN Jun-xia QIN Kui SU Shuai ZHAO Peng CUI Zhi-zhong |
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| Institution: | 1. Animal Medical College of Shandong Agricultural University, Tai'an 271018, China;2. Institute of Animal Health Supervision of Fushan District, Yantai City, Yantai 265500, China |
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| Abstract: | In a certain area of Shandong province, Marek's disease (MD) occurred in diseased chickens that had been vaccinated by turkey herpesvirus.In order to isolate the virus strain and detect the virus pathogenicity, agar diffusion test, cell culture and indirect immunofluorescence assay (IFA) were used to isolate the Marek's virus from chicken's blood and feather marrow.The isolated strain was adapted to grow in chick embryo fibroblasts (CEF).Genes involved in pathogenesis of MDV, such as meq, pp38 and 132 bp repeat sequence were amplified by PCR.The obtained sequences were compared with that of standard strains published in GenBank by DNAStar software.The results showed that pp38 gene of the SDAU-1 shared homology from 100% with standard virulent sequence.Analysis of 132 bp repeat sequence and meq gene sequences of the viral genome showed that the isolated virus belongs to the highly virulent MDV strains. |
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| Keywords: | Marek's disease virus isolation identification sequence comparison meq gene |
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