Cloning of Mx Gene of Beijing Duck and its Expression Analysis in DEF Incubated with Duck Reovirus |
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| Authors: | DING Ming-yang GHEN Zong-yan WU Run LIU Guang-qing |
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| Institution: | 1. Shanghai Veterinary Research Institute, CAAS, Shanghai 200241, China;2. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China |
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| Abstract: | The study was aimed to research the structure and function of Mx gene in Beijing duck.Full-length sequence of Beijing duck Mx gene was amplified by RT-PCR from the total RNA extracted from duck embryo fibroblast(DEF) induced by Poly(I:C).Furthermore, the expression of Mx gene in DEF infected with DRV was described.Sequence analysis indicated that the duck Mx gene contained an open reading frame(ORF) of 2166 bp encoding a protein of 721 amino acids.A phylogenetic tree based on Mx gene sequence was constructed.The results showed that Beijing duck Mx gene had the lowest distance with the gene from wild duck available in GenBank.Homology analysis showed that Beijing duck Mx gene nucleotides and deduced amino acids shared 47.8% to 99.8% and 47.9% to 99.4% homologies with those from other animals available in GenBank, respectively.Fluctuation expression of Beijing duck Mx gene was found with DEF incubated with duck reovirus.Duck Mx gene was successfully cloned and predicted with characteristic of typical structure of Mx family.Gaining Beijing duck Mx gene laid a foundation for further researching Mx protein antiviral activity, molecular mechanism and interferon monitoring of poultry. |
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| Keywords: | Mx gene Beijing duck antiviral activity duck reovirus |
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