Everolimus prevents experimental IgA nephropathy in rats |
| |
| Authors: | ZHOU Min TANG Hui-ling |
| |
| Institution: | Pharmaceutical College, Jiangsu Food & Pharmaceutical Science College, Huai'an 223003, China |
| |
| Abstract: | AIM: To investigate the effects of everolimus on the experimental IgA nephropathy in rats and its possible mechanisms.METHODS: The rat model of experimental IgA nephropathy was established. The rats were randomly divided into control group, IgA group and everolimus treatment group. After the corresponding treatments were given, urinary red blood cells, protein and N-acetyl-β-D-glucosaminidase (NAG) were examined. Immunofluorescence staining was used to analyze the level of IgA precipitation in the renal tissues. Additionally, the protein expression of myeloid differentiation factor 88 (MyD88), TLR4, NF-κB, IL-4 and IL-13 was determined by Western blot. The mRNA levels of IL-4 and IL-13 were detected by qPCR.RESULTS: Everolimus significantly inhibited the increases in the urinary levels of red blood cells, protein and NAG in experimental IgA nephropathy rats. Furthermore, IgA nephropathy-induced increases in the protein expression of MyD88, TLR4, NF-κB, IL-4 and IL-13 were attenuated after everolimus treatment. Similar results were obtained in the mRNA levels of IL-4 and IL-13 by qPCR detection.CONCLUSION: Everolimus improves the impairments of renal function in experimental IgA nephropathy rats as evidenced by decreasing urinary red blood cells, protein and NAG, which may be related to the inhibition of MyD88, TLR4, NF-κB, IL-4 and IL-13 expression. |
| |
| Keywords: | Everolimus IgA nephropathy Myeloid differentiation factor 88 NF-κB TLR4 IL-4 IL-13 |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|
