Effect of Different Weaning Age on Related-growth Gene Expressions of Spleen in Piglets |
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| Authors: | YANG Jing-jing LI Yuan LI Liu-an JIN Tian-ming JIAO Xiao-li |
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| Institution: | 1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China;2. Animal Husbandry Post-doctoral Station, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | In order to study the effect of different weaning age on related-growth gene expressions of spleen in piglets,24 similar body weight and healthy piglets were selected (Duroc×Landrace×Yorkshine).All piglets were randomly divided into 4 groups (14,21,28 and 35 days of age weaning groups),and each group had 6 replications including 1 piglet separately.They were slaughtered at 24 days of age and spleen samples were acquired.The relative expression of growth hormone receptor (GHR),insulin like growth factor-1 (IGF-1),insulin like growth factor-1 receptor (IGF-1R) mRNA in spleen were measured by RT-PCR.The results showed that the relative expression of GHR mRNA in 14 and 21 days of age weaning groups was significantly higher than that in 35 days of age weaning group (P<0.05);The relative expression of IGF-1 mRNA in spleen at different weaning age groups was no significant difference (P>0.05);The relative expression of IGF-1R mRNA in 35 days of age weaning group was significantly higher than that in 28 days of age weaning group (P<0.05).The results indicated that the patterns of weaning age on related growth gene expressions of spleen are different;The expressions of spleen IGF-1R mRNA in different weaning age groups were possibly effected by the spleen self regulation of IGF-1. |
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| Keywords: | weaning age piglet spleen gene expression GHR IGF-1 IGF-1R |
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