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| 非洲紫罗兰花瓣细胞pH值测定及F3‘5’H基因片段克隆 | |
| 引用本文： | 裴仁济,陈小强,孙宁,张乃楠,刘阳,刘峰华,张磊.非洲紫罗兰花瓣细胞pH值测定及F3‘5’H基因片段克隆[J].天津农学院学报,2012,19(1):11-14. |
| 作者姓名： | 裴仁济 陈小强 孙宁 张乃楠 刘阳 刘峰华 张磊 |
| 作者单位： | 1. 天津农学院农学系,天津300384／天津天士力现代中药资源有限公司资源发展部,天津300410 2. 天津农学院农学系,天津,300384 3. 天津天士力现代中药资源有限公司资源发展部,天津,300410 |
| 基金项目： | 天津市科技支撑计划重点项目“非洲紫罗兰新品种的引进和育种”，天津市应用基础及前沿技术研究计划资助项目“兰花子房启动发育相关基因的克隆与表达” |
| 摘 要： | 以白色和蓝色品种的非洲紫罗兰舌状花瓣为材料，测定其细胞液pH值，并利用RT-PCR技术，以NCBI上登记的马铃薯、矮牵牛、龙胆草、金鱼草和瓜叶菊的F3‘5＇H基因片段保守区域为模板，克隆乃芍，爿基因片段，为花色显色机理研究提供理论依据。实验结果表明：蓝色品种非洲紫罗兰细胞液的pH值大于白色品种非洲紫罗兰花瓣细胞液的pH值，随着花期的延长，两个品种非洲紫罗兰细胞液的pH值变化不断增大，且白色品种非洲紫罗兰花瓣细胞液的pH值变化大于蓝色品种非洲紫罗兰花瓣细胞液的pH值；其范围均在3．0-7．0之间，呈弱酸；在两种花色品种非洲紫罗兰中，都得到了F3‘5＇H基因片段的cDNA序列长度为392bp，与马铃薯、矮牵牛、龙胆草、金鱼草和瓜叶菊的F3‘5＇H基因保守区域片段的同源性分别为63．O％、55．4％、52．8％、49．0％和47．7％。 |
| 关 键 词： | 非洲紫罗兰 RT-PcR技术 F3＇5＇H基因 pH值 花色 |
Petal Cell pH Value Determination and F3‘5＇H Gene Fragment Cloning of Saintpqulia ionantha |
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| PEI Ren-ji,CHEN Xiao-qiang,SUN Ning,ZHANG Nai-nan,LIU Yang,LIU Feng-hua,ZHANG Lei.Petal Cell pH Value Determination and F3‘5＇H Gene Fragment Cloning of Saintpqulia ionantha[J].Journal of Tianjin Agricultural College,2012,19(1):11-14. | |
| Authors: | PEI Ren-ji CHEN Xiao-qiang SUN Ning ZHANG Nai-nan LIU Yang LIU Feng-hua ZHANG Lei |
| Institution: | 1 (1.Department of Agronomy,Tianjin Agricultural University,Tianjin 300384,China;2.Department of Resource Development, Tianshili Modern Chinese Medicine Resource Co.Limited,Tianjin 300410,China) |
| Abstract: | The experiment used white and blue Saintpqulia ionantha cultivars’ linguoid petal as test materials to determine their pH values of cellular sap and clone F3’5’H gene fragment which was compared with the F3’5’H genes of Solanum tuberosum, Petunia hybrida,gentiana scabra bge,Snapdragon,Pericallis cruentia that were registered in NCBI by using the RT-PCR technique for the study of coloration mechanism.The results indicate that the pH value in blue Saintpqulia ionantha cultivar’ cellular sap was obviously higher than that in white Saintpqulia ionantha cultivar’ cellular sap,and the pH values of two Saintpqulia ionantha cultivars petal cell showed increasing trend,the changes of pH value were greater in white Saintpqulia ionantha cultivar’ cellular sap than those in blue Saintpqulia ionantha cultivar’ cellular sap.And the range of their pH values was between 3.0 and 7.0,which was faintly acid.In two Saintpqulia ionantha cultivars,the cDNA sequence of F3’5’H gene fragment were both 392 bp.Compared with the F3’5’H genes,conservative area of Solanum tuberosum,Petunia hybrida,gentiana scabra bge,Snapdragon,Pericallis cruentia,the homology of nucleotide sequence separately reached 63.0%,55.4%,52.8%,49.0%and 47.7%. |
| Keywords: | Saintpaulia ionantha RT-PCR technology F3'5'H gene pH value flower color |
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