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| 生长相关的微卫星标记在镜鲤繁殖群体中的验证 | |
| 引用本文： | 鲁翠云,曹顶臣,李超,李崇文,孙效文.生长相关的微卫星标记在镜鲤繁殖群体中的验证[J].天津农学院学报,2012(3):13-18. |
| 作者姓名： | 鲁翠云 曹顶臣 李超 李崇文 孙效文 |
| 作者单位： | 1. 中国水产科学研究院 黑龙江水产研究所,哈尔滨 150070 2. 天津市天祥水产有限责任公司,天津 301500 3. 上海海洋大学 水产与生命学院,上海 201306 |
| 基金项目： | 农业部“引进国际先进农业科学技术”计划项目“水产主导品种分子育种技术引进”(2011-G12);公益性行业(农业)科研专项“水产分子育种共性技术的建立与应用”(200903045);国家高技术研究发展计划项目“鲤生长等经济性状的主效基因发掘及育种潜力评估”(2011AA00402) |
| 摘 要： | 利用大样本镜鲤繁殖群体分析和验证8个生长相关微卫星标记与体质量和体长的相关性：结果显示：8个微卫星标记在镜鲤繁殖群体中均获得了稳定、清晰的DNA条带，片段大小在100-322bp之间，共检测到33个等位基因、75种基因型。对每个微卫星座位的基因型与体质量、体长性状进行相关性分析发现，HLJ343与体质量、体长显著相关；HLJ319、HLJ302及HLJE339与体长显著相关。本研究用大样本随机群体证实了此4个QTL位点与体质量或体长性状具有相关性，下一步可根据优势基因型指导家系配组，开展基于QTL结果的分子聚合育种研究。 |
| 关 键 词： | 微卫星 体重 体长 镜鲤 |
Verification of Microsatellite Markers Correlated with Growth Trait in Mirror Carp Cyprinus carpio L. |
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| LU Cui-yun,CAO Ding-chen,LI Chao,LI Chong-wen,SUN Xiao-wen.Verification of Microsatellite Markers Correlated with Growth Trait in Mirror Carp Cyprinus carpio L.[J].Journal of Tianjin Agricultural College,2012(3):13-18. | |
| Authors: | LU Cui-yun CAO Ding-chen LI Chao LI Chong-wen SUN Xiao-wen |
| Institution: | 1,Corresponding Author(1.Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China;2.Tianjin Tianxiang Aquatic Company Limited,Tianjin 301500,China;3.College of Fisheries and life Science,Shanghai Ocean University,Shanghai 201306,China) |
| Abstract: | Correlation between Genotypes of eight microsatellite loci and growth trait was analyzed and verified using mirror carp breeding population with large samples.Steady and clear DNA bands were amplified in 8 microsatellite markers with the size ranged from 100 to 322 bp.Thirty-three alleles and 75 genotypes were detected.The correlation was analyzed using data of body weight,body length and genotypes.The results show that HLJ343 had a significant impact on body weight and length.In addition,there were three loci,HLJ319,HLJ302 and HLJE339,and they had a significant impact on body length.This analysis reveals that the 4 QTL loci had correlation with body weight and length in large samples of mirror carp.Next,it can guide the breeding program according to the dominant genotype.And then,the researches in molecular marker assistant breeding selection will be carried out based on the QTL results. |
| Keywords: | microsatellite marker body weight body length Cyprins carpio L |
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