| 不同授粉技术对克服百合杂交受精前障碍的影响 |
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| 引用本文： | 温韦华,张克中,贾月慧,杨雪珍.不同授粉技术对克服百合杂交受精前障碍的影响[J].北京农学院学报,2010,25(4):32-36. |
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| 作者姓名： | 温韦华 张克中 贾月慧 杨雪珍 |
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| 作者单位： | 北京农学院园林学院,组培中心,北京102206;北京农学院园林学院,组培中心,北京102206;北京农学院园林学院,组培中心,北京102206;北京农学院园林学院,组培中心,北京102206 |
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| 基金项目： | 北京市科技新星项目，北京市教委科技创新平台项目 |
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| 摘 要： | 研究柱头直接授粉法、柱头直接授粉＋切花柱授粉法、柱头直接授粉＋花柱嫁接法等技术对克服百合杂种系内及杂种系间受精前杂交障碍的影响。结果发现：所试验的几个杂种系内杂交组合中,柱头直接授粉法能使各组合结果率均达到100%。所试验的几个杂种系间杂交组合中,柱头直接授粉法不能促使父本花粉管进入子房;柱头直接授粉＋切花柱授粉法能促使部分花粉管进入子房,但定向转向珠孔端的花粉管不多,该方法使亚洲百合（♀）×东方百合3个组合中获得果实及少量种子;柱头直接授粉＋花柱嫁接法使亚洲百合（♀）×东方百合组合有少量花粉管进入子房,并可能发生向珠孔端的定向生长,采用离体子房切片-胚珠培养后,有一个组合获得1株小苗。
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| 关 键 词： | 百合 柱头直接授粉法 切花柱授粉法 花柱嫁接法 花粉管定向生长 |
The Effects of Different Pollination Techniques on Overcoming the Pre-fertilization Barriers in lilium Breeding |
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| WEN Wei-hua,ZHANG Ke-zhong,JIA Yue-hui,YANG Xue-zhen.The Effects of Different Pollination Techniques on Overcoming the Pre-fertilization Barriers in lilium Breeding[J].Journal of Beijing Agricultural College,2010,25(4):32-36. |
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| Authors: | WEN Wei-hua ZHANG Ke-zhong JIA Yue-hui YANG Xue-zhen |
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| Institution: | WEN Wei-hua,ZHANG Ke-zhong ,JIA Yue-hui,YANG Xue-zhen(Landscape Design & Forestry College,Center of Tissue Culture,Beijing University of Agriculture,Beijing102206,China) |
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| Abstract: | The effects of different pollination techniques such as stigma pollination method,Stigma pollination+cut style pollination method and Stigma pollination+ graft style pollination method on overcoming lily’s pre-fertilization cross barriers were investigated.The results showed as follows : of the tested intra-hybrid swarm,fruitset was 100% respectively by using stigma pollination method.of the tested inter-hybrid swarm,female pollen tubes were not able to enter ovary by using stigma pollination method;while some female pollen tubes were observed to enter ovary and few of them turned to the micropylars via using Stigma pollination+Cut style pollination method,fruits and few of seeds were gained in 3 combinations of Asian Lily(♀)×Oriental lily by using this pollination method;Within the 3 tested intra-section cross combinations of Asian Lily(♀)×Oriental lily,while using Stigma pollination+ graft style pollination method,few of pollen tubes might achieve directional growth to the micropylars and one hybrid seedling was acquired in vitro ovule-slice culture and ovule culture. |
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| Keywords: | lilium stigma pollination method cut style pollination method graft style pollination method pollen tubes’ directional growth |
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