| 丁草胺降解菌的分离鉴定及降解特性的研究 |
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| 引用本文： | 楚小强,庞国辉,方华,王秀国,高春明,虞云龙.丁草胺降解菌的分离鉴定及降解特性的研究[J].农业环境科学学报,2009,28(1). |
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| 作者姓名： | 楚小强 庞国辉 方华 王秀国 高春明 虞云龙 |
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| 作者单位： | 浙江大学农业与生物技术学院植物保护系,浙江杭州,310029 |
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| 基金项目： | 浙江省科技厅项目，浙江省自然科学基金 |
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| 摘 要： | 从处理农药生产废水的膜生物反应器中分离到一株能以丁草胺为惟一碳源和能源生长的细菌BD-1,经鉴定为施氏假单孢菌(Pseudomonas stutzeri).在纯培养的条件下测定了BD-1对丁草胺的降解性能.结果表明,在接种量为菌浓度OD415=0.2,pH7.0、30℃条件下,BD-l对丁草胺的降解符合一级动力学特征,1.0、10.0和100.0 rag·L的丁草胺的降解半衰期分别为0.11、0.60和0.96d.BD-1在不同pH及温度下对丁草胺的降解作用为pH 7.0>pH6.0>pH 8.0,30℃>20℃>40℃.GC/MS初步分析结果表明,丁草胺的主要微生物降解产物为2-氯-2',6'-二乙基乙酰苯胺和2,6-二乙基苯胺.
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| 关 键 词： | 丁草胺 施氏假单孢菌 生物降解 |
Isolation, Identification and Characteristics of a Butachlor-degrading Bacterium |
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| CHU Xiao-qiang,PANG Guo-hui,FANG Hua,WANG Xiu-guo,GAO Chun-ming,YU Yun-long.Isolation, Identification and Characteristics of a Butachlor-degrading Bacterium[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2009,28(1). |
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| Authors: | CHU Xiao-qiang PANG Guo-hui FANG Hua WANG Xiu-guo GAO Chun-ming YU Yun-long |
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| Institution: | Department of Plant Protection;College of Agriculture and Biotechnology;Zhejiang University;Hangzhou 310029;China |
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| Abstract: | A bacterial strain BD-1 capable of utilizing butachlor as sole carbon and energy sources was isolated from the sludge of a mem brane bioreactor after successive enrichment cultures. The isolated strain BD-1 was identified as Pseudomonas stutzeri based on its morphol ogy, physiological and biochemical characteristics, Biolog GN2 analysis together with the 16S rDNA homologue sequence analysis. The degradation characteristics of butachlor by this strain were investigated in liquid culture. The degradation half... |
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| Keywords: | butachlor pseudomonas stutzeri biodegradation |
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