Role of exogenous glutathione in teleost fish and its effects on antioxidant defense responses in rainbow trout exposed to 3,3',4,4'-tetrachlorobiphenyl |
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| Authors: | DME Otto CK Sen N Hidiroglou R Madere TW Moon |
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| Institution: | (1) Ottawa-Carleton Institute of Biology, Department of Biology, University of Ottawa, Ottawa, ON, K1N 6N5, Canada;(2) Department of Molecular and Cell Biology, University of California, 251 Life Sciences Addition, Berkeley, CA94720, USA;(3) Department of Physiology, Faculty of Medicine, University of Kuopio, Finland;(4) Nutrition Research Division, Health Canada, Ottawa, ON, K1A 0L2, Canada |
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| Abstract: | Intraperitoneal injection of glutathione (GSH) is shown to effectively increase tissue glutathione content in two teleost species, the rainbow trout and the American eel. GSH uptake into teleost tissues is a distinct piscine feature of GSH biochemistry compared with mammalian species where little or not uptake occurs of exogenously added GSH. Enhancement and depletion of tissue GSH status was used as a tool to examine the effects of altered GSH content on other cellular antioxidants in 3,3 ,4,4-tetrachlorobiphenyl (TCB) exposed rainbow trout.  -Tocopherol content was unchanged and thiobarbituric acid reactive substances (TBARS), an indicator of lipid peroxidation, changed little in liver and muscle of trout exposed to TCB and the various GSH treatments. However,  -tocopherol levels in muscle and superoxide dismutase activities in the various tissues were significantly higher in controls and TCB-saline treated fish than in GSH deficient and lipoate supplemented TCB exposed trout. GSH status thus appears to affect some cellular antioxidant defenses in TCB exposed trout. |
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| Keywords: | glutathione supplementation glutathione biochemistry antioxidant enzymes rainbow trout American eel |
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