| 牙鲆生长抑素基因的表达及细胞定位 |
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| 引用本文： | 陈晓武,施志仪,江山,李倩.牙鲆生长抑素基因的表达及细胞定位[J].水产学报,2012,36(4):497-502. |
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| 作者姓名： | 陈晓武 施志仪 江山 李倩 |
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| 作者单位： | 上海海洋大学农业部淡水水产种质资源重点实验室,上海,201306 |
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| 基金项目： | 国家自然科学基金项目(31172392); 上海市重点学科水生生物学建设项目(S30701); 上海市教委科研创新项目(12YZ126) |
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| 摘 要： | 采用RACE和荧光定量PCR技术克隆牙鲆生长抑素(SS)基因cDNA序列并且分析了其在仔鱼期的表达规律,同时通过免疫细胞化学染色,对牙鲆胃肠道和脑垂体等器官的生长抑素分泌细胞进行定位。最终获取包含完整编码区的牙鲆SS基因cDNA序列,其编码区长为381 bp,推测编码的蛋白质为126个氨基酸,其前23个氨基酸为信号肽,分子量为14.3 ku,理论等电点为8.33。牙鲆仔鱼不同发育时期的SS基因表达量比较结果表明,出膜后仔鱼体内SS基因表达不断增加,到变态发育期变化不大,变态后期又开始显著增加。免疫组织化学染色结果表明,SS细胞存在于胃、胰腺和脑垂体中。在胃中,生长抑素分泌细胞主要分布于胃上皮层;在胰腺中,生长抑素分泌细胞零散地分布,血管周围较多;在脑垂体中细胞密度高,集中在腺垂体的周边区域。
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| 关 键 词： | 牙鲆 生长抑素 序列分析 免疫组化 |
| 收稿时间： | 2011/9/14 0:00:00 |
| 修稿时间： | 2011/10/22 0:00:00 |
Expression and cellular localization of somatostatin from Paralichthys olivaceus |
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| CHEN Xiao-wu,SHI Zhi-yi,JIANG Shan and LI Qian.Expression and cellular localization of somatostatin from Paralichthys olivaceus[J].Journal of Fisheries of China,2012,36(4):497-502. |
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| Authors: | CHEN Xiao-wu SHI Zhi-yi JIANG Shan and LI Qian |
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| Institution: | (Key Laboratory of Freshwater Aquatic Genetic Resources,Shanghai Ocean University,Ministry of Agriculture,Shanghai 201306) |
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| Abstract: | Somatostatin (SS) called also somatotropin release-inhibiting factor is a peptide named for its ability to inhibit pituitary growth hormone release. To facilitate investigation of the SS function in the teleost, its coding sequence in paralichthys olivaceus was isolated by the technique of Rapid Amplification of cDNA Ends (RACE). SYBR Green I Quantitative Real-time PCR analysis and immunohistochemistry were used to assess the expression of SS at mRNA and protein level. The deduced protein sequence contains 126 amino acid residues and a predicted molecular mass of 14.3 kD. Very low level of SS mRNA is presented during the first three days after hatching. The expression level of the SS gene was increased constantly before metamorphosis and steadily during the metamorphosis, then increased significantly during post-metamorphosis. There was no SS-positive reaction in the oropharyngeal cavity and esophagus. SS secreting cells are only distributed in the stomach, pancreas and pituitary. Several types of SS secreting cells could be distinguished in above organs. In stomach, SS secreting cells are dispersively distributed among the epithelium cells, presenting various kinds of forms such as ovoid, globular, triangle, elongated or spindle-shaped aspects. The pancreas and liver of flounder combine into a single organ named hepatopancreas, and SS-positive cells are only located in the pancreas, showing as globular-shaped, most of them are closed to blood vessels. SS-positive cells at high density are located in pars distalis of pituitary, so it is difficult to distinguish the shape of those cells. |
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| Keywords: | Paralichthys olivaceus somatostatin sequence analysis immunohistochemistry |
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