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内蒙古驼蹄瓣属植物亲缘关系的RAPD分析
引用本文:张辰波,史雪松,宛涛,曹艳伟,张晓明.内蒙古驼蹄瓣属植物亲缘关系的RAPD分析[J].中国草地学报,2006,28(5):86-90.
作者姓名:张辰波  史雪松  宛涛  曹艳伟  张晓明
作者单位:1. 内蒙古农业大学农学院,内蒙古,呼和浩特,010019
2. 海拉尔学院,内蒙古,海拉尔,025000
摘    要:应用RAPD技术对七种驼蹄瓣属植物及其近缘种霸王的亲缘关系进行了研究,16个引物共扩增出125条带,其中99条表现出多态性,占79.2%,表现出丰富的RAPD多态性;利用UPGMA构建了分子系统树,结果表明RAPD分析的亲缘关系聚类基本上与经典分类相一致,表明RAPD技术适用于驼蹄瓣属植物亲缘关系的研究.

关 键 词:驼蹄瓣属  RAPD  亲缘关系  霸王
文章编号:1673-5021(2006)05-0086-05
修稿时间:2006年3月4日

Studies on the Genetic Relationships of 7 Species of Zygophyllum in Inner Mongolia Based on Random Amplified Polymorphic DNA (RAPD)
ZHANG Chen-bo,SHI Xue-song,WAN Tao,CAO Yan-wei,ZHANG Xiao-ming.Studies on the Genetic Relationships of 7 Species of Zygophyllum in Inner Mongolia Based on Random Amplified Polymorphic DNA (RAPD)[J].Chinese Journal of Grassland,2006,28(5):86-90.
Authors:ZHANG Chen-bo  SHI Xue-song  WAN Tao  CAO Yan-wei  ZHANG Xiao-ming
Abstract:Genetic relationship of 7 species of Zygophyllum L.and S.xanthoxylon Bunge were analysed by RAPD markers with 16 primers.125 bands were amplified,99(79.2%) of which were polymorphic.It showed that Zygophyllum has high genetic diversity.Molecular systematic tree constructed with UPGMA indicated that the results of clustering analysis based on RAPD was consistent with those based on traditional methods,which indicated that RAPD molecular marker could be used for genetic relationship researches on Zygophyllum.
Keywords:RAPD
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