| 云南甘蔗宿根矮化病病原检测* |
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| 引用本文： | 黄应昆；李文凤；赵,俊；饶,云；卢文洁；李,俊；罗志明；杨洪昌.云南甘蔗宿根矮化病病原检测*[J].云南农业大学学报,2007,22(5):762-765. |
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| 作者姓名： | 黄应昆；李文凤；赵 俊；饶 云；卢文洁；李 俊；罗志明；杨洪昌 |
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| 作者单位： | 云南省农业科学院甘蔗研究所 云南开远661600(黄应昆，李文凤，赵俊，卢文洁，李俊，罗志明，杨洪昌)，云南省新平县农业局 云南新平653400(饶云) |
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| 基金项目： | 国家“948”项目(2003-Q06)，云南省重大农业推广项目(2005) |
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| 摘 要： | 利用从澳大利亚引进的甘蔗宿根矮化病病原（RSD）的标准抗原抗体，建立了I-ELISA检测甘蔗宿根矮化病的方法。采用电镜负染法和I-ELISA法，对采自云南红河、开远、新平蔗区的42个样本进行RSD检测，结果表明：29个样本为阳性，13个样本为阴性，确认云南蔗区存在RSD。
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| 关 键 词： | 云南 甘蔗宿根矮化病 发生 检测 |
| 文章编号： | 1004-390X（2007）05-0762-04 |
| 收稿时间： | 2006-10-17 |
| 修稿时间： | 2006-10-17 |
Primary Pathogen Detection of Sugarcane Ratoon Stunting Disease in Yunnan |
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| HUANG Ying-kun,LI Wen-feng,ZHAO Jun,RAO Yun,LU Wen-jie,LI Jun,LUO Zhi-ming,YANG Hong-chang.Primary Pathogen Detection of Sugarcane Ratoon Stunting Disease in Yunnan[J].Journal of Yunnan Agricultural University,2007,22(5):762-765. |
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| Authors: | HUANG Ying-kun LI Wen-feng ZHAO Jun RAO Yun LU Wen-jie LI Jun LUO Zhi-ming YANG Hong-chang |
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| Institution: | Sugarcane Research Institute, Yunnan Academy of Agricultural Science, Kaiyuan 661600, China ; Agricultural Bureau of Xinping county, Yunnan Province Xinping 653400,China |
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| Abstract: | The mean of I-ELISA of detecting pathogen of RSD is established based on standard antigen and antibody from Australia.42 samples from Honghe,Kaiyuan and Xinping were detected by means of negative staining of electron microscope and I-ELISA.The results indicated that 29 samples are positive and 13 samples are negative,so as to prove that RSD occur in Yunnan. |
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| Keywords: | Yunnan sugarcane ratoon stunting disease occurrence detection |
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