| 辣椒分子连锁遗传图谱的构建及抗疫病QTL定位 |
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| 引用本文： | 安静,胡勇胜,张宝玺,毛胜利,王立浩.辣椒分子连锁遗传图谱的构建及抗疫病QTL定位[J].中国蔬菜,2007,1(10):9-12. |
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| 作者姓名： | 安静 胡勇胜 张宝玺 毛胜利 王立浩 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所，100081 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 以抗疫病材料perennial和感疫病材料83-60杂交得到的F2群体144个单株为试材,利用AFLP、RAPD、SSR等分子标记,采用JoinMap3.0构建辣椒分子连锁遗传图谱,该图谱包括12个连锁群,70个标记位点,其中AFLP标记54个,RAPD标记15个,SSR标记1个,覆盖长度为429.02cM,平均图距为6.13cM,连锁群长度在4.30～85.85cM之间。对F2群体进行辣椒疫病的室内人工接种,利用Windows QTL Cartographer2.0软件进行QTL分析,在第4连锁群上检测到两个QTL,解释表型差异的贡献率分别为9.5%和16.4%。
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| 关 键 词： | 辣椒 遗传图谱 抗疫病 QTL |
| 收稿时间： | 2007-05-08; |
| 修稿时间： | 2007-05-08 |
Genetic Linkage Map Construction and QTL Analysis for Phytophthora capsici L. in Pepper |
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| An Jin , Hu Yongsheng, Zhang Baoxi ,et al..Genetic Linkage Map Construction and QTL Analysis for Phytophthora capsici L. in Pepper[J].China Vegetables,2007,1(10):9-12. |
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| Authors: | An Jin Hu Yongsheng Zhang Baoxi |
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| Institution: | Institute of Vegetables and Flowers; Chinese Academy of Agricultural Sciences; Beijing 100081; College of Horticulture and Landscape; Hunan Agricultural University |
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| Abstract: | The F2 population including 144 individuals used for this research was derived from the resistance inbred line Perennial and the sensitive inbred line 83-60.A genetic linkage map of pepper was constructed based on this F2 population by using JoinMap 3.0.This map consists of 12 main linkage groups,covers 429.02 cM,includes 54 AFLP markers,15 RAPD markers and 1 SSR marker.The average interval distance is 6.13 cM,each linkage group distance is 4.30-85.85 cM.Two QTLs identified on group 4 are significant resistance to Phytophthora capsici L.that accounted for 9.5 % and 16.4 % of the total phenotypic variance. |
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| Keywords: | QTL |
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