| 荔枝幼胚的离体培养 |
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| 引用本文： | 吕柳新,余小玲.荔枝幼胚的离体培养[J].福建农林大学学报(自然科学版),1993(4). |
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| 作者姓名： | 吕柳新 余小玲 |
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| 作者单位： | 福建农学院园艺系 350002 |
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| 摘 要： | 把3个不同胚胎发育类型荔枝(Litchi chinensis Sonn.)品种乌叶、兰竹、绿荷包的幼胚置于含有不同生长调节剂的MS培养基上进行培养。结果表明:授粉后15d的胚胎人工培养十分困难,而授粉后30-40d的幼胚,在MS基本培养基附加一定浓度的BA,NAA,GA以及ABA等的不同组合培养基上,均可诱导形成细胞团,并能进一步形成胚状体或芽状体,部分败育型品种兰竹,在胚胎败育早期但未死亡的幼胚,以及完全败育型品种绿荷包,在授粉后30d的胚珠,经培养亦能形成胚状体,胚状体在MS或1/2MS附加低浓度的细胞分裂素及生长素等的培养基上,增殖能力甚强,甚至不加任何附加物的情况下亦能增殖。高浓度的附加物则不利于胚状体的增殖。从胚状体诱导成苗有待进一步研究。
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| 关 键 词： | 荔枝 幼胚 离体培养 胚状体 |
Culture of the Immature Embryo of Litchi in Vitro |
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| Lu Liuxin,Yu Xiaoling Dept of Horticulture,FAU,Fuzhou.Culture of the Immature Embryo of Litchi in Vitro[J].Journal of Fujian Agricultural and Forestry University,1993(4). |
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| Authors: | Lu Liuxin Yu Xiaoling Dept of Horticulture FAU Fuzhou |
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| Institution: | Lu Liuxin,Yu Xiaoling Dept of Horticulture,FAU,Fuzhou 350002 |
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| Abstract: | Immature embryos of 3 litchi cultivars(Wuye,Lanzhu and Luhebao,belonging to 3 types of embryonic development,respectively)were cultured on MS media supplemented with growth regulators.The results showed that it is very difficulty to culture litchi immature embryos 15 d after pollination.But most of embryos 30-40 d after pollination could be induced into cell aggregate on MS media containing BA,NAA, GA and ABA,and further develop into embryoids and bud-like bodies.Embryoids were also induced from the immature embryos of partly abortive cv.Lanzhu in the early period of abortion but not being died,and ovules picked up 30 d after pollination from completely abortive cv.Luhebao.The multiplication capability of embryoids was very strong when they were cultured on MS or 1/2 MS media supplemented with the low level of cytokinin and other growth regulators,even multiplicated on the MS media without any growth regu- lator.High level supplements inhibited the multiplication of embryoids.Further development of embryoids into plantlets should be further studied. |
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| Keywords: | litchi immature embryo in vitro culture embryoid |
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