| 蚕蛹壳几丁质降解菌的分离筛选及其产酶条件优化 |
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| 引用本文： | 赵祥杰,邝哲师,叶明强,罗国庆,肖更生,廖森泰.蚕蛹壳几丁质降解菌的分离筛选及其产酶条件优化[J].安徽农业科学,2011(5):2807-2809,2812. |
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| 作者姓名： | 赵祥杰 邝哲师 叶明强 罗国庆 肖更生 廖森泰 |
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| 作者单位： | 广东省农业科学院农业生物技术研究所;广东省农业科学院蚕业与农产品加工研究所; |
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| 基金项目： | 广东省农业科学院科技支撑项目(07支-撑-04); 广东省农业攻关计划项目(2007A0201000043); 国家现代农业产业技术体系建设专项资金资助 |
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| 摘 要： | 目的]对产几丁质酶菌株发酵产酶活性工艺进行优化。方法]将筛选得到的1株产几丁质酶菌株G-254进行驯化培养,通过单因素试验考察了不同碳源、氮源和无机盐对菌株产酶活的影响;进行响应面试验,以菌株发酵所产酶活为响应值,确定最佳的发酵产酶工艺条件。结果]菌株G-254发酵产几丁质酶最佳发酵条件为,葡萄糖8%,牛肉膏料5%,硫酸镁0.07%,在此条件下获得的几丁质酶活为6.86 U。结论]提高了菌株发酵产几丁质酶酶活,为后续工业化发酵生产奠定了基础。
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| 关 键 词： | 蚕蛹壳 几丁质 降解 酶活 优化 |
Isolation and Screening of Silkworm Puparium Chitin Degradation Strains and the Optimization of Chitinase-produce Conditions |
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| ZHAO Xiang-jie et al.Isolation and Screening of Silkworm Puparium Chitin Degradation Strains and the Optimization of Chitinase-produce Conditions[J].Journal of Anhui Agricultural Sciences,2011(5):2807-2809,2812. |
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| Authors: | ZHAO Xiang-jie |
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| Institution: | ZHAO Xiang-jie et al (Agricultural Biotechnology Research Institute of Guangdong Academy of Agricultural Sciences,Sericulture & Farm Produce Processing Research Institute of Guangdong Academy of Agricultural Sciences,Guangzhou,Guangdong 510610) |
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| Abstract: | Objective] The aim of this study was to optimize the conditions of chitinase-produce strains.Method] A kind of screened chitinase-produce strain G-254 was habituated cultured,and then the single factor experiment was carried out to explore the effects of different carbon source,nitrogen source and inorganic salt on the activity of produced chitinase;the response surface test was used to determine the optimal conditions for chitinase production.Result] The optimal conditions for chitinase production were:... |
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| Keywords: | Silkworm puparium Chitin Degradation Chitinase activity Optimization |
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