| 黄鳝抗菌肽hepcidin基因大肠杆菌表达载体的构建 |
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| 引用本文： | 刘成都,邱庆崇,耿晓雪,李伟.黄鳝抗菌肽hepcidin基因大肠杆菌表达载体的构建[J].安徽农业科学,2011(9):5245-5246. |
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| 作者姓名： | 刘成都 邱庆崇 耿晓雪 李伟 |
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| 作者单位： | 长江大学生命科学学院; |
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| 基金项目： | 长江大学2009年大学生创新性实验计划项目(0901) |
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| 摘 要： | 目的]构建黄鳝抗菌肽hepcidin基因大肠杆菌表达载体。方法]根据Genbank中黄鳝hepcidin基因序列设计引物进行PCR扩增,将测序正确的基因片段连接入载体pET-28a中。结果]以黄鳝cDNA为模板,扩增出hepcidin基因片段,长度为291 bp,连接到pET-28a上。经PCR扩增、双酶切验证,证实成功构建原核表达载体。结论]成功构建了黄鳝hepcidin基因大肠杆菌表达载体。
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| 关 键 词： | 黄鳝 抗菌肽 基因 原核表达载体 |
Constructing of A E. coli Expression Vector of Anti-bacterial Peptides Hepcidin Gene from Eel |
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| LIU Cheng-du et al.Constructing of A E. coli Expression Vector of Anti-bacterial Peptides Hepcidin Gene from Eel[J].Journal of Anhui Agricultural Sciences,2011(9):5245-5246. |
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| Authors: | LIU Cheng-du |
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| Institution: | LIU Cheng-du et al(College of Life Science at Yangtze University,Jingzhou,Hubei 434025) |
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| Abstract: | Objective]To construct a E.coli expression vector of hepcidin gene from rice field eel.Method]According to hepcidin gene sequence of rice field eel reported by genbank,primers were designed,and PCR was performed to amplify the hepcidin gene.Then the purified fragment was cloned into pET-28a vector.Result] Hepcidin gene was amplified from the cDNA of rice field eel.The gene fragment was 291 bp,and was cloned into pET-28a vector.After analysis of restriction endonuclease digestion and PCR,the constructed e... |
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| Keywords: | Rice field eel Anti-bacterial peptides Hepcidin Gene E coli expression vector |
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