| 大黄药材中大黄苷元含量测定 |
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| 引用本文： | 宿爱山,王淑美,梁生旺,李建生,王战红.大黄药材中大黄苷元含量测定[J].安徽农业科学,2011(11):6382-6383,6518. |
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| 作者姓名： | 宿爱山 王淑美 梁生旺 李建生 王战红 |
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| 作者单位： | 广东药学院;河南中医学院; |
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| 基金项目： | 国家自然科学基金项目(81073024); 广东省自然科学基金项目(9451022401003860); 广东药学院重点培养教师资助计划 |
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| 摘 要： | 目的]采用HPLC法测定不同产地的大黄药材中5种蒽醌苷元的含量。方法]HPLC色谱条件为：色谱柱为Diamonsil C18柱（5μm,250.0 mm×4.6 mm）;流动相为甲醇-0.05%磷酸水溶液（V∶V=85∶15）;流速为1 ml/min;检测波长245 nm;进样量为20μl。结果]大黄各苷元线性关系良好,平均回收率为97.55%～100.87%,RSD值为1.38%～2.47%。大黄药材中各苷元含量范围分别为：芦荟大黄素0.26%～0.52%,大黄酸0.21%～1.47%,大黄素0.26%～0.40%,大黄酚0.29%～1.95%,大黄素甲醚0.48%～2.72%。结论]该方法精密度良好、重现性、稳定性好,适合大黄药材中各大黄苷元的质量控制。
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| 关 键 词： | 大黄 蒽醌类苷元 含量测定 HPLC法 |
Determination of Anthraquinones Aglycone in RADIX ET RHIZOMA RHEI |
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| SU Ai-shan et al.Determination of Anthraquinones Aglycone in RADIX ET RHIZOMA RHEI[J].Journal of Anhui Agricultural Sciences,2011(11):6382-6383,6518. |
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| Authors: | SU Ai-shan |
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| Institution: | SU Ai-shan et al(Guangdong Pharmaceutical University,Guangzhou,Guangdong 510006) |
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| Abstract: | Objective] To determine contents of five anthraquinones aglycones by HPLC.Method] HPLC conditions were as follows: Diamonsil C18 column(4.6 mm×250.0 mm,5 μm),mobile phase of methanol-0.05% phosphoric acid(V∶ V=85∶ 15),flow rate of 1 ml/min,detection wavelength of 245 nm and sampling amount of 20 μl.Result] Good in linear relationship,the recovery rate of each aglycone ranged from 97.55%-100.87% with RSD value of 1.38%-2.47%.Contents of aloe-emodin,rhein,emodin,chrysophanol and physcion in RADIX ET RHIZOMA RHEI were: 0.26%-0.52%,0.21%-1.47%,0.26%-0.40%,0.29%-1.95% and 0.48%-2.72%,respectively.Conclusion] Good in precison,repeatability and stability,the method established in this study favored the quality control of each aglycone in RADIX ET RHIZOMA RHEI. |
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| Keywords: | RADIX ET RHIZOMA RHEI Anthraquinones aglycone Determination of content HPLC |
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