| 猪饲料用乳酸菌增菌培养基的优化研究 |
| |
| 引用本文： | 杜刚,田孟华,杨海英,杨胜江.猪饲料用乳酸菌增菌培养基的优化研究[J].安徽农业科学,2011(9):5259-5260,5262. |
| |
| 作者姓名： | 杜刚 田孟华 杨海英 杨胜江 |
| |
| 作者单位： | 云南民族大学化学与生物技术学院民族药资源化学国家民委-教育部重点实验室; |
| |
| 基金项目： | 云南省教育厅科学研究基金项目(08Z0045); 云南民族大学青年基金科研项目(09QN08) |
| |
| 摘 要： | 目的]通过对培养基成分及pH值的研究,得到最佳乳酸菌增菌培养基。方法]采用pH值梯度培养确定培养基的最适pH值;采用PB试验确定培养基主要影响因子;采用响应面试验对乳酸菌培养基进行优化,得出影响因子间的相互关系,并得到最佳培养基。结果]适于乳酸菌培养的最适起始pH值为6.8;牛肉膏、葡萄糖和NaAc为乳酸菌增殖的主要影响因子,牛肉膏是最重要的影响因子;优化后的最佳培养基:牛肉膏23.70 g/L、葡萄糖47.53 g/L、NaAc4.87 g/L、蛋白胨10.0 g/L、酵母膏10.0 g/L、柠檬酸二胺2.0 g/L、Mn-SO4 0.2 g/L、吐温80 0.5 g/L。结论]优化后的乳酸菌增菌培养基使活菌密度显著提高,达到制备乳酸菌菌剂需求。
|
| 关 键 词： | 乳酸菌 增菌培养基 优化 菌体密度 |
Optimization of Lactic Acid Bacteria Culture Conditions |
| |
| DU Gang et al.Optimization of Lactic Acid Bacteria Culture Conditions[J].Journal of Anhui Agricultural Sciences,2011(9):5259-5260,5262. |
| |
| Authors: | DU Gang |
| |
| Institution: | DU Gang et al(Key Laboratory of Ethnic Medicine Resource Chemistry,State Ethnic Affairs Commission & Ministry of Education,College of Chemistry and biotechnology,Yunnan University of Nationalities,Kunming,Yunnan 650031) |
| |
| Abstract: | Objective] The optimum enrichment medium of lactic-acid bacteria was optimized for the lactic-acid bacteria inoculants production of pig feed preparation by the study of pH and medium component.Method] The optimum pH of medium was determined with pH gradient culture.The main effect factors were determined with Plackett-Burrman(PB) design.The optimum enrichment medium and the interrelationship were achieved through Response Surface Methodology(RSM).Result]The results showed that the optimum pH6.8,the main... |
| |
| Keywords: | Lactic-acid bacteria Enrichment medium Medium optimization Cell density |
| 本文献已被 CNKI 等数据库收录！ |
