| 大片段DNA提取和纯化的优化方案 |
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| 引用本文： | 陈琰,艾云灿.大片段DNA提取和纯化的优化方案[J].安徽农业科学,2011,39(9):5096-5097,5100. |
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| 作者姓名： | 陈琰 艾云灿 |
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| 作者单位： | 中山大学生命科学学院,广东广州,510275;中山大学生命科学学院,广东广州,510275 |
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| 基金项目： | 中国大洋矿产资源研究开发协会资助项目(DYXM-115-02.2.14) |
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| 摘 要： | 目的]研究大片段DNA提取和纯化的优化方案研究。方法]在传统SDS提取法的基础上,对大片段DNA的提取和纯化过程进行了改进。结果]建立了一种优化的DNA提取和纯化的方案,通过该方案能够得到片段大、纯度高的DNA。结论]为今后大片段DNA的提取和纯化提供了参考。
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| 关 键 词： | 大片段DNA 提取 纯化 优化方案 |
The Optimal Protocol of Extraction and Purification of Large Fragment DNA |
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| CHEN Yan et al.The Optimal Protocol of Extraction and Purification of Large Fragment DNA[J].Journal of Anhui Agricultural Sciences,2011,39(9):5096-5097,5100. |
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| Authors: | CHEN Yan |
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| Institution: | CHEN Yan et al(School of Life Science,Sun Yat-sen University,Guangzhou,Guangdong 510275) |
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| Abstract: | Objective] The aim was to study the optimum proposal of big segment DNA extraction and purification.Method] Based on traditional SDS extraction,the extraction and purification process of big segment DNA were improved.Result] A optimized scheme of DNA extraction and purification was established,based on this scheme,DNA with big segment and high purity were obtained.Conclusion] All those provided references for big segment DNA extraction and purification in the future. |
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| Keywords: | Big segment DNA Extraction Purification Optimized scheme |
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