| 兜兰宽瓣亚属8种植物的核型比较 |
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| 引用本文： | 杨志娟,朱根发,吕复兵,张显,王碧青.兜兰宽瓣亚属8种植物的核型比较[J].园艺学报,2006,33(5):1015-1019. |
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| 作者姓名： | 杨志娟 朱根发 吕复兵 张显 王碧青 |
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| 作者单位： | ( 广东省农业科学院花卉研究所, 广东广州510640; 西北农林科技大学园艺学院, 陕西杨凌712100) |
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| 基金项目： | 广东省科技攻关重大专项资助项目(2003A2010401) |
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| 摘 要： | 对兜兰宽瓣亚属( Paphiopedilum subgenus brachypetalum ) 8种植物的染色体数目和核型进行比较研究。结果表明: 供试的兜兰原生种的染色体数目均为26, 二倍体。白花兜兰( P. emersonii) 2n = 2x =26 = 18m + 8sm, 核型不对称系数59190%; 同色兜兰( P. concolor) 2n = 2x = 26 = 2M + 18m + 4sm + 2st, 核型不对称系数56.90%; 巨瓣兜兰( P. bellatulum ) 2n = 2x = 26 = 22m + 2sm + 2st, 核型不对称系数56.10%; 汉氏兜兰( P. hangianum ) 2n = 2x = 26 = 22m + 4sm, 核型不对称系数58.64%; 麻栗坡兜兰(P. m alipoense) 2n = 2x = 26 = 20m + 6sm, 核型不对称系数59.41%; 浅斑兜兰( P. jackii) 2n = 2x = 26 =14m + 10sm + 2st, 核型不对称系数63.00%; 杏黄兜兰( P. arm eniacum ) 2n = 2x = 26 = 24m + 2sm, 核型不对称系数55.05%; 硬叶兜兰( P. micranthum ) 2n = 2x = 26 = 20m + 6sm, 核型不对称系数56.91%。除同色兜兰、巨瓣兜兰和汉氏兜兰的核型类型为“2A”外, 其它为“2B”。染色体长度变化不明显, 主要由中部着丝粒染色体和近中部着丝粒染色体组成, 未见随体结构。这些核型特征为兜兰属植物的系统进化提供了细胞分类学的理论依据。
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| 关 键 词： | 兰科 兜兰属 宽瓣亚属 染色体数目 核型 |
| 文章编号： | 0513-353X（2006）05-1015-06 |
| 收稿时间： | 2005-12-26 |
| 修稿时间： | 2005-12-262006-04-17 |
Studies on the Karyotypes of Eight Species of Paphiopedilum subgenus brachypetalum |
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| Yang Zhijuan,Zhu Genfa,Lü Fubing,Zhang Xian,Wang Biqing.Studies on the Karyotypes of Eight Species of Paphiopedilum subgenus brachypetalum[J].Acta Horticulturae Sinica,2006,33(5):1015-1019. |
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| Authors: | Yang Zhijuan Zhu Genfa Lü Fubing Zhang Xian Wang Biqing |
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| Institution: | 1.Liwan Hospital, Guangzhou Medical College, Guangzhou 510170, China;2.Key Laboratory of Ophthalmology of The Ministry of Education, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China |
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| Abstract: | Eight species of Paphiopedilum subgenus brachypetalum were karyologically analyzed by root tips squash method. The result showed that the number of the somatic chromosome of these plants is 2n = 26, diploid. The karyotypes were as follows: P. emersonii 2n = 2x = 26 = 18m + 8 sm, index of the karyotypic asymmetry (As.k) = 59.90%; P. concolor 2n = 2x = 26 = 2M + 18m + 4sm + 2st, As.k = 56.90%; P.bellatulum 2n = 2x = 26 = 22m + 2sm + 2st, As.k = 56.10%; P. hangianum 2n = 2x = 26 = 22m + 4 sm, As.k = 58.64%; P. malipoense 2n = 2x = 26 = 20m + 6sm, As.k = 59.41%; P. jackii 2n = 2x = 26 = 14m+ 10sm + 2st, As.k = 63.00%; P. armeniacum 2n = 2x = 26 = 24m + 2sm, As.k = 55.05%; P. micranthum 2n = 2x = 26 = 20m + 6sm, As.k = 56.91%. According to Stebbins classification of karyotypic asymmery, the karyotype of P. concolor, P. bellatulum and P. hangianum belonged to “2A”, the others belonged to “2B”. The diploid mainly consisted of metacentric and submetacentric chromosomes, but not satellites. Moreover, results of this karyological study provided some references on cytotaxonomy for system evolution of Paphiopedilum. |
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| Keywords: | Orchidaceae Paphiopedilum Subgenus brachypetalum Chromosome number Karyotype |
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