| 简单重复序列(AAG)_5在百萨偃麦草染色体上的分布 |
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| 引用本文： | 温辉芹,裴自友,任永康.简单重复序列(AAG)_5在百萨偃麦草染色体上的分布[J].山西农业科学,2011,39(6):505-507,511. |
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| 作者姓名： | 温辉芹 裴自友 任永康 |
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| 作者单位： | 山西省农业科学院作物科学研究所,山西太原,030032 |
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| 基金项目： | 山西省自然科学基金项目 |
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| 摘 要： | 百萨偃麦草是小麦亲缘物种之一,对环境胁迫和生物胁迫具有很强的抗性,是小麦遗传改良的重要资源。为了对导入的外源染色体及片段进行有效鉴定,利用荧光原位杂交方法,研究了合成的简单重复序列(AAG)5在百萨偃麦草染色体上的分布情况。结果表明,(AAG)5在百萨偃麦草上有多个位点,利用(AAG)5作探针可以将百萨偃麦草的各条染色体区分开来。
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| 关 键 词： | 简单重复序列 百萨偃麦草 荧光原位杂交(FISH) |
Distribution of (AAG)5 Sequence on Chromosomes of Thinopyrum bessarabicum |
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| WEN Hui-qin,PEI Zi-you,REN Yong-kang.Distribution of (AAG)5 Sequence on Chromosomes of Thinopyrum bessarabicum[J].Journal of Shanxi Agricultural Sciences,2011,39(6):505-507,511. |
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| Authors: | WEN Hui-qin PEI Zi-you REN Yong-kang |
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| Institution: | WEN Hui-qin,PEI Zi-you,REN Yong-kang(Institute of Crop Sciences,Shanxi Academy of Agricultural Sciences,Taiyuan 030032,China) |
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| Abstract: | Thinopyrum bessarabicum Lve is one of wheat relatives with high tolerance to both biotic and abiotic stresses.FISH techniques were used for locating the synthetic oligonucleotide（AAG）5 sequence to specific chromosomes of Thinopyrum bessarabicum chromosomes.The results showed that there were many distinctive signals distribution sites of simple sequence repeats（AAG）5 on the chromosomes,and using（AAG）5 as probe,each chromosome of Thinopyrum bessarabicum could be well distinguished. |
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| Keywords: | simple sequence repeats（SSRs） Thinopyrum bessarabicum Lve fluorescence in situ hybridization（FISH） |
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