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土壤酶活性对土壤中土霉素的动态响应
引用本文:姚志鹏,李兆君,梁永超,张永清,姚建华,解晓瑜.土壤酶活性对土壤中土霉素的动态响应[J].植物营养与肥料学报,2009,15(3):696-700.
作者姓名:姚志鹏  李兆君  梁永超  张永清  姚建华  解晓瑜
作者单位:1.农业部作物营养与施肥重点实验室,中国农业科学院农业资源与农业区划研究所,北京 100081;
基金项目:国家自然科学基金,International Foundation for Science;through a grant to zhaojun Li,中央级公益性科研院所基本科研业务费专项基金,中国农业科学院农业资源与农业区划研究所所长基金 
摘    要:采用室内培养试验研究了土霉素(OTC)对土壤过氧化氢酶、磷酸酶、脲酶、蔗糖酶和脱氢酶活性的影响。结果表明,在整个培养期间,土霉素对土壤过氧化氢酶和土壤磷酸酶活性具有明显的抑制作用;对土壤蔗糖酶和脱氢酶活性在培养前期具有轻微的抑制作用,但培养后期(培养第112 d)具有较强的抑制作用;而对土壤脲酶活性的影响则相反,在培养第1 d,OTC 100 mg/kg处理对脲酶具有显著的刺激作用,以后土霉素对脲酶活性影响不明显。土壤过氧化氢酶和磷酸酶对土霉素污染响应比土壤脲酶、蔗糖酶和脱氢酶更敏感,因而可以表征土壤受土霉素的污染程度。

关 键 词:土霉素    土壤酶活性    黑土
收稿时间:2008-3-11

Dynamic responses of soil enzymatic activities to oxytetracycline in soil
YAO Zhi-peng,LI Zhao-jun,LIANG Yong-chao,ZHANG Yong-qing,YAO Jian-hua,XIE Xiao-yu.Dynamic responses of soil enzymatic activities to oxytetracycline in soil[J].Plant Nutrition and Fertilizer Science,2009,15(3):696-700.
Authors:YAO Zhi-peng  LI Zhao-jun  LIANG Yong-chao  ZHANG Yong-qing  YAO Jian-hua  XIE Xiao-yu
Institution:1.Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization/Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China;
Abstract:Effects of oxytetracycline (OTC) on soil enzymes including soil catalase, phosphatase, urease, sucrase, and dehydrogenase activities were studied under laboratory incubation conditions in the present paper. During the entire period of incubation, the activities of catalase, and phosphatase were significantly inhibited by OTC added. The inhibition of activities of soil sucrase and dehydrogenase by OTC was dependent on the retention time of OTC in soil. The activities of soil sucrase and dehydrogenase were slightly decreased until 56 days after OTC application (DAA), and were significantly decreased at 122 DAA. The adverse effect of OTC on soil urease was also noted. The activity of soil urease was siginificantly stimulated by OTC at the rate of 100 mg/kg added at 1 DAA, however, no significant effects of OTC on soil urease were observed during the remaining period of incubation time. Soil catalase and phosphatase were more sensitive to OTC in soil than other soil enzymes such as soil urease, sucrase, and dehydrogenase. Therefore, soil catalase and phosphatase can indicate soil contamination caused by OTC.
Keywords:oxytetracycline  soil enzymatic activities  black soil
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