Growth activity of bovid herpesvirus 1 in bovine follicular oocytes with cumulus cells. |
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| Authors: | T Tsuboi Y Kanazawa T Syoji S Tokuhisa |
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| Institution: | Tohoku Branch Laboratory, National Institute of Animal Health, Aomori, Japan. |
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| Abstract: | Bovine follicular oocytes collected from bovine ovaries were exposed to bovid herpesvirus 1 (BHV-1). After washings, these oocytes were cultured to mature. As a result BHV-1 could not be removed from the oocytes and could replicate in the oocytes with cumulus cells, but not in the oocytes without the cells. Moreover, the specific fluorescence for BHV-1 was detected in the cumulus cells by a indirect immunofluorescent technique. Therefore these findings suggested BHV-1 could be absorbed in the oocytes but the replication of BHV-1 was done in the cumulus cells. |
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