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| 小麦黄花叶病毒河南驻马店分离物的鉴定与全序列分析 | |
| 引用本文： | 张宗英,徐建美,韩成贵,张振臣,李大伟,于嘉林.小麦黄花叶病毒河南驻马店分离物的鉴定与全序列分析[J].华北农学报,2010,25(2). |
| 作者姓名： | 张宗英 徐建美 韩成贵 张振臣 李大伟 于嘉林 |
| 作者单位： | 1. 中国农业大学,植物病理系,北京,100193;中国农业大学,农学与生物技术国家重点实验室,北京,100193 2. 河南省农业科学院,植物保护研究所,河南,郑州,450002 3. 中国农业大学,农学与生物技术国家重点实验室,北京,100193 |
| 摘 要： | 采集河南省驻马店市13个不同田块小麦样品,利用RT-PCR、ELISA和Western Blotting检测技术进行检测,结果表明,该地有小麦黄花叶病毒的存在与分布。对检测为阳性的一个分离物全序列进行cDNA克隆和测序,与已报道WYMV河南潢川、江苏扬州、四川雅安和日本分离物的全序列进行比对,RNA1核苷酸一致性为97.1%~98.4%;RNA2为95.1%~98.4%;氨基酸一致性分别为94.8%~97.8%和94.2%~98.2%。利用软件MEGA4.1对上述五个RNA1和RNA2的全序列分别进行进化树分析,结果表明,驻马店分离物与潢川亲缘关系最近,而与四川雅安亲缘关系最远。 |
| 关 键 词： | 小麦黄花叶病毒 驻马店分离物 病毒检测 基因组全序列 |
Detective and Complete Sequence Analysis of Wheat Yellow Mosaic Virus from Zhumadian in Henan Province |
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| ZHANG Zong-ying,XU Jian-mei,HAN Cheng-gui,ZHANG Zhen-chen,LI Da-wei,YU Jia-lin.Detective and Complete Sequence Analysis of Wheat Yellow Mosaic Virus from Zhumadian in Henan Province[J].Acta Agriculturae Boreali-Sinica,2010,25(2). | |
| Authors: | ZHANG Zong-ying XU Jian-mei HAN Cheng-gui ZHANG Zhen-chen LI Da-wei YU Jia-lin |
| Institution: | ZHANG Zong-ying1,2,XU Jian-mei1,HAN Cheng-gui1,ZHANG Zhen-chen3LI Da-wei2,YU Jia-lin2(1.Department of Plant Pathology,China Agricultural University,Beijing 100193,China,2.State Key Laboratory for Agrobiotechnology,3.Institute of Plant Protection,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China) |
| Abstract: | A total of 13 wheat samples from different fields in Zhumadian of Henan Province were investigated,using RT-PCR,ELISA and Western Blotting,the results showed that WYMV existed there.Complete RNA genomic cDNA of one positive isolate(WYMV-ZMD)was cloned and sequenced,and was compared with those of isolates from Huangchuan,Yangzhou,Ya'an and Japan.Sequence comparisons showed that WYMV-ZMD shared 97.1%-98.4% nucleotide sequence identity for RNA1 with the reported isolates,and 94.2%-98.2% identity for RNA2.At th... |
| Keywords: | Wheat yellow mosaic virus Zhumadian isolate Detection Complete sequence |
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