Genetic diversity of Castanopsis acuminatissima (Bl.) A. DC. in northern Thailand and the selection of seed trees for forest restoration |
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| Authors: | Blakesley David Pakkad Greuk James Celia Torre Franck Elliott Stephen |
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| Institution: | (1) Horticulture Research International, East Malling, West Malling, Kent, ME19 6BJ, UK (e-mail;(2) Forest Restoration Research Unit, Biology Department, Science Faculty, Chiang Mai University, 50200, Thailand |
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| Abstract: | Castanopsis acuminatissima (Bl.) A. DC. is one of a number of  framework species which are being planted to restore seasonally dry tropical forests in northern Thailand. This study describes the level of microsatellite variation within and among three populations of this species in three National Parks in northern Thailand: Doi Suthep-Pui, Doi Inthanon and Jae Sawn, using published primers developed for Castanopsis cuspidata var. sieboldii Nakai. The five microsatellite loci employed in this study detected a total of 54 alleles (n = 72). The informativeness of the microsatellite loci varied from six to 18 alleles, with an average of 10.8 alleles found over all loci. The mean observed heterozygosities in the three populations showed no significant deviations from Hardy-Weinberg expectations. The vast majority of genetic diversity was contained within the populations, with no significant differentiation between them (FST = 0.006). Algorithms were designed to capture microsatellite diversity, and the rationale for using microsatellite markers to inform genetic conservation is discussed. The implications for seed collection of C. acuminatissima for forest restoration are also discussed. |
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| Keywords: | Castanopsis acuminatissima Forest restoration Genetic diversity Microsatellites Tropical trees |
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