| 一个新的抗玉米矮花叶病基因的发现及初步定位 |
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| 引用本文： | 席章营,张书红,李新海,谢传晓,李明顺,郝转芳,张德贵,梁业红,白丽,张世煌.一个新的抗玉米矮花叶病基因的发现及初步定位[J].作物学报,2008,34(9):1494-1499. |
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| 作者姓名： | 席章营 张书红 李新海 谢传晓 李明顺 郝转芳 张德贵 梁业红 白丽 张世煌 |
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| 作者单位： | 1.中国农业科学院作物科学研究所, 北京100081;2.河南农业大学农学院, 河南郑州450002 |
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| 基金项目： | 中国博士后科学基金，引进国际先进农业科技基金(948计划) |
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| 摘 要： | 由SCMV引起的矮花叶病是我国的主要玉米病害之一, 鉴定和发掘新的抗病基因对于玉米抗病遗传育种具有重要意义。以抗病自交系海9-21和感病自交系掖478杂交的一个BC2F3群体为试验材料, 通过人工接种矮花叶病毒进行抗病性鉴定, 发现该分离群体中抗病植株与感病植株数符合1∶3的分离比例, 推测其抗病基因是由1对隐性基因控制。抗感池和SSR标记连锁分析表明, 存在一个新的玉米矮花叶病隐性抗病基因(或等位基因), 将该基因命名为scm3。scm3基因来源于抗病玉米自交系海9-21, 位于第3染色体短臂3.04~3.05区域, 在SSR标记umc1965和bnlg420之间, 遗传距离分别为45.7 cM和6.5 cM。连锁的标记还有umc1307、umc2265、bnlg2241和umc2166, 它们与scm3之间的遗传距离分别是8.3、13.3、15.5和19.7 cM, 这些SSR标记与scm3基因在染色体上的排列顺序为umc1965—scm3—bnlg420—umc1307—umc2265—bnlg2241—umc2166。
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| 关 键 词： | 玉米矮花叶病 甘蔗花叶病毒 抗病基因 分子标记 基因定位 |
| 收稿时间： | 2007-11-16 |
| 修稿时间： | 1900-01-01 |
Identification and Mapping of a New Resistant Gene to Sugarcane Mosaic Virus in Maize |
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| XI Zhang-Ying,ZHANG Shu-Hong,LI Xin-Hai,XIE Chuan-Xiao,LI Ming-Shun,HAO Zhuan-Fang,ZHANG De-Gui,LIANG Ye-Hong,BAI Li,ZHANG Shi-Huang.Identification and Mapping of a New Resistant Gene to Sugarcane Mosaic Virus in Maize[J].Acta Agronomica Sinica,2008,34(9):1494-1499. |
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| Authors: | XI Zhang-Ying ZHANG Shu-Hong LI Xin-Hai XIE Chuan-Xiao LI Ming-Shun HAO Zhuan-Fang ZHANG De-Gui LIANG Ye-Hong BAI Li ZHANG Shi-Huang |
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| Institution: | 1.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081;2.College of Agronomy, Henan Agricultural University, Zhengzhou 450002, Henan, China |
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| Abstract: | Sugarcane mosaic virus (SCMV) causes considerable damage to maize (Zea mays L.) in China. Understanding the genetic of resistance gene(s) is the basis for effective selection in resistance breeding program. The objective of the present study was to identify the new resistance gene to SCMV in China maize germplasm. Twenty-one BC2F3 populations derived from eight maize intercross combinations were planted in field trials under artificial inoculation. The number of resistant and susceptible plants in one BC2F3 population (Ye 478 ´ Hai 9-21) was found to be in accordance with the theoretical 1:3 ratio．A recessive gene derived from the resistant parent Hai 9-21 was supposed to control the resistance to SCMV. With the aid of BSA (bulked segregant analysis) and SSR marker analyses, a new recessive resistance gene to maize SCMV was identified, designated as scm3. The scm3 gene was located in the bin3.04/05, flanked by the SSR markers umc1965 and bnlg420 with the genetic distances of 45.7 and 6.5 cM, respectively. The other linked markers include umc1307, umc2265, bnlg2241, and umc2166. These linked markers are in the order of umc1965–scm3–bnlg420–umc1307–umc2265–bnlg2241–umc2166. Resistant germplasm to SCMV in maize was en-riched with the identification and mapping of scm3. |
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| Keywords: | Maize dwarf mosaic SCMV Resistance gene Molecular marker Gene mapping |
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