| 茶藨生柱锈重寄生木霉粗毒素的基本性质 |
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| 引用本文： | 李永艳,敖新宇,陈玉惠.茶藨生柱锈重寄生木霉粗毒素的基本性质[J].天津农业科学,2011,17(2):11-15. |
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| 作者姓名： | 李永艳 敖新宇 陈玉惠 |
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| 作者单位： | 1. 西南林业大学保护生物学学院,云南昆明,650224
2. 西南林业大学基础部,云南昆明,650224 |
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| 基金项目： | 西南林业大学重点基金，云南省森林灾害预警与控制重点实验室资助项目 |
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| 摘 要： | 采用液体发酵和生物测定相结合的方法,研究了茶藨生柱锈(Cronartium ribicola)重寄生木霉SS003菌株(Trichoderma atroviride)所产粗毒素的基本性质。结果表明,SS003所产粗毒素为一类非蛋白质、极性较大的物质,具有较高的热稳定性和酸碱稳定性,能被甲醇较好地提取出来,可被活性碳有效地吸附并为甲醇所洗脱。该结果为SS003菌株的活性成分研究及进一步开发利用奠定基础。
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| 关 键 词： | 木霉 粗毒素 基本性质 茶藨生柱锈 |
Elementary Properties of Crude Toxin Produced by Mycoparasite (Trichoderma atroviride) of Cronartium ribicola |
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| LI Yong-yan,AO Xin-yu,CHEN Yu-hui.Elementary Properties of Crude Toxin Produced by Mycoparasite (Trichoderma atroviride) of Cronartium ribicola[J].Tianjin Agricultural Sciences,2011,17(2):11-15. |
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| Authors: | LI Yong-yan AO Xin-yu CHEN Yu-hui |
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| Institution: | LI Yong-yan1,AO Xin-yu2,CHEN Yu-hui2(1.Faculty of Conservation Biology,Southwest Forestry University,Kunming,Yunnan 650224,China,2.Department of Fundamental Courses,China) |
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| Abstract: | Adopting the methods of liquid culture and biologic detection,the elementary properties of crude toxin produced by mycoparasite(Trichoderma atroviride) SS003 of Cronartium ribicola were studied.The results showed that the crude toxin produced by SS003 was a kind of non-protein material with higher polarity.The produced toxin,which could be tolerant to high temperature and pH value variations,could be fully extracted by methanol,the toxin was effectively absorbed by the activated charcoal and be eluted by me... |
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| Keywords: | Trichoderma spp crude toxin elementary property Cronartium ribicola |
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