| 黄瓜黑星病菌致病机理的研究Ⅱ细胞壁降解酶及其在致病中的作用 |
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| 引用本文： | 李宝聚,陈红漫.黄瓜黑星病菌致病机理的研究Ⅱ细胞壁降解酶及其在致病中的作用[J].植物病理学报,2000,30(1):13-18. |
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| 作者姓名： | 李宝聚 陈红漫 |
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| 作者单位： | 1. 辽宁省农业科学院植物保护研究所, 沈阳 110161;2. 沈阳农业大学酶工程中心, 沈阳 110161 |
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| 基金项目： | 辽宁省科委重点科技攻关资助项目(94201032) |
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| 摘 要： | 本文通过活体内外黄瓜黑星病菌(Cladosporium cucumerinum)产生的细胞壁降解酶活性分析,初步明确了Cx-酶(羧甲基纤维素酶)、β-葡萄糖苷酶和PMG(聚甲基半乳糖醛酸酶)、PMTE(果胶甲基反式消除酶)在该菌侵染黄瓜中的作用。瓜条感病的各部位,以病健交界处细胞壁降解酶活性最高;在检测的各类酶中,活性最高的为PMG、PMTE,Cx-酶、β-葡萄糖苷酶和FPA(滤纸酶)活性较低。抗感病黄瓜品种染病后,细胞壁降解酶活性均迅速增强。感病品种染病后,果胶酶(PMG、PMTE)活性远远高于抗病品种,其高峰值是抗病品种的2.72~7.52倍。感病品种接种后纤维素酶(Cx-酶、β-葡萄糖苷酶)活性一直增强;抗病品种接种后β-葡萄糖苷酶随着接种天数的增加酶活性一直增强,Cx-酶在染病后初期活性迅速增强,增至高峰后逐渐降低。
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| 关 键 词： | 黄瓜 黑星病菌 细胞壁降解酶 |
| 文章编号： | 0412-0914(2000)01-0013-06 |
| 修稿时间： | 1998-05-07 |
PATHOGENIC
MECHANISM OF SCAB OF CUCUMBER CAUSED
BY Cladosporium cucumerinum |
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| LI Bao ju,ZHOU Chang li,ZHAO Kui hua,LI Feng yun.PATHOGENIC
MECHANISM OF SCAB OF CUCUMBER CAUSED
BY Cladosporium cucumerinum[J].Acta Phytopathologica Sinica,2000,30(1):13-18. |
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| Authors: | LI Bao ju ZHOU Chang li ZHAO Kui hua LI Feng yun |
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| Institution: | Enzyme Center, Shenyang Agricultural University, Shenyang 110161, China |
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| Abstract: | Activities of the cell wall degrading enzymes (CWDEs) produced by C.cucumerinum both in vitro and in vivo were studied.The results primarily demonstrated that Cx, β glucosidase, PMG and PMTE played roles during the infection.Among each part of diseased cucumber, the activity of the CWDEs around the boundary between diseased and healthy areas was the highest.For all kinds of CWDEs that could be examined, the activities of the PMG andPMTE were higher, and those of Cx and β glucosidase were relatively lower.Activities of CWDEs in susceptible and resistant cultivars were rapidly improved after inoculation.However, activities of the pectinase(PMG, PMTE) in susceptible cultivars were significantly higher than those in resistant cultivars.The peak values in the former were 2.72-7.52 times of those in the latter.After inoculation, activities of the cellulase (Cx, β glucosidase) increased consistently in susceptible cultivars.The activity of β glucosidase increased as the disease development in resistant cultivars, while activity of Cx only increased during early days, and gradually decreased when that reached the peak however. |
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| Keywords: | Cucumber Cladosporium cucumerinum cell wall degrading enzymes |
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