| 黄瓜种质资源遗传多样性的AFLP分析 |
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| 引用本文： | 张桂华,韩毅科,杨瑞环,杜胜利,王鸣.黄瓜种质资源遗传多样性的AFLP分析[J].华北农学报,2007,22(3):21-24. |
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| 作者姓名： | 张桂华 韩毅科 杨瑞环 杜胜利 王鸣 |
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| 作者单位： | 1. 天津科润黄瓜研究所,天津,300192
2. 西北农林科技大学,陕西,杨凌,712100 |
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| 基金项目： | 国家高技术研究发展计划(863计划)
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天津市农业科学院院长基金 |
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| 摘 要： | 对23份不同来源的黄瓜材料进行了AFLP分析。18对引物组合共扩增出543条谱带,其中,多态性带为106条,多态率为19.5%。UPGA分类结果将23份黄瓜材料划分为三大类群:第1类群为野生型黄瓜的聚类;第2类群为美国类型黄瓜、荷兰类型黄瓜以及具有荷兰血统的黄瓜的聚类;第3类群中包含所有中国类型黄瓜和2个前苏联类型黄瓜。遗传相似性分析和聚类结果表明:野生黄瓜和栽培黄瓜之间的亲缘关系较远,AFLP标记的分类结果与材料的主要性状特点基本一致。
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| 关 键 词： | 黄瓜 AFLP 遗传多样性 |
| 文章编号： | 1000-7091（2007）03-0021-04 |
| 修稿时间： | 2006-11-29 |
AFLP Analysis of Genetic Diversity of Cucumber Germplasm |
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| ZHANG Gui-hua,HAN Yi-ke,YANG Rui-huan,DU Sheng-li,WANG Ming.AFLP Analysis of Genetic Diversity of Cucumber Germplasm[J].Acta Agriculturae Boreali-Sinica,2007,22(3):21-24. |
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| Authors: | ZHANG Gui-hua HAN Yi-ke YANG Rui-huan DU Sheng-li WANG Ming |
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| Institution: | 1.Tianjin Cucumber Research Institute, Tianjin 300192, China; 2. Northwest Science and Technology University of Agriculture and Forestry, Yangling 712100, China |
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| Abstract: | The genetic diversity of 23 cucumber materials were analyzed by AFLP technique.543 DNA bands were amplified by 18 pairs of AFLP primers,of which there were 106 polymorphic bands,and the percentage of polymorphic bands was 19.5%.The dendrogram of 23 cucumber materials was generated by UPGMA(Unweighred Pair-group Method Arithmetic Average) method,and was divided into 3 groups.The first group was the clustering of wide species,the second group included stateside type,Netherlandish type,and the type possessed the origin of Netherland.The third group included all of the Chinese type materials and two Russian type materials.The genetic similarity and cluster analysis showed that the relationship between wild germplasms and cultivars was far,and the classification result based on AFLP markers was basically consistent with that on their morphological characters. |
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| Keywords: | Cucumber AFLP Genetic diversity |
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