| 赤霉素负调控因子GhRGL(RGL-LIKE)基因序列与功能预测分析 |
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| 引用本文： | 廖文彬,崔百明,温玮,彭明.赤霉素负调控因子GhRGL(RGL-LIKE)基因序列与功能预测分析[J].热带作物学报,2009,30(2):170-175. |
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| 作者姓名： | 廖文彬 崔百明 温玮 彭明 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所,海口,571101 |
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| 基金项目： | 国家重点基础研究发展规划(973计划) |
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| 摘 要： | 海岛棉(Gossypium barbadense L.)的产量与激素相关,根据基因序列的同源性,笔者首次从海岛棉中克隆到1个类似赤霉素(GA)信号途径中的负调控因子DELLA蛋白基因,并对该基因的序列进行分析.结果表明:该基因序列包含有DELLA蛋白所有的保守结构域,如DELLA结构域、VHYNP结构域、多聚Poly S/T保守区、2个亮氨酸拉链、1个GRAS结构域、1个核定位信号和1个VHVID保守区:其蛋白序列与拟南芥中的5个DELLA蛋白GA Insensitive(GAI)、Repressor of gal-3(RGA)、RGLI(RGA-like 1)、RGL2、RGL3氨基酸序列的相似度分别为60.5%、59.8%、63.1%、61.8%和58.1%.特将其定义为GhRGL.对GhRGL基因的5'序列进行克隆并利用Plantcare进行元件预测,发现该基因启动子存在着大量与激素相关的调控元件,暗示该基因的功能可能与激素调控有关.利用ProtScale程序对该蛋白进行疏/亲水性分析,初步确定该蛋白质的抗原决定簇的位置,为该基因的抗体制备奠定了基础.
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| 关 键 词： | 海岛棉 GhRGL基因 DELLA蛋白 序列分析 功能预测 |
Sequence of GhRGL (RGL-LIKE) Gene and Its Bioinformatics Analysis |
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| Liao Wenbin,Cui Baiming,Wen Wei and Peng Ming.Sequence of GhRGL (RGL-LIKE) Gene and Its Bioinformatics Analysis[J].Chinese Journal of Tropical Crops,2009,30(2):170-175. |
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| Authors: | Liao Wenbin Cui Baiming Wen Wei and Peng Ming |
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| Institution: | Liao Wenbin,Cui Baiming,Wen Wei,Peng Ming Institute of Tropical Bioscience , Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China |
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| Abstract: | Yield of sea island cotton (Gossypium barbadense L.) is related with hormone. A DELLA protein gene similar to a negative regulatory element in the message path of GA was first isolated from the sea island cotton, and its sequences were analyzed by bioinformatics. All conserved domains of DELLA protein in Arabidopsis were found in this protein, such as DELLA domain and VHYNP domain, poly S/T region, two LXXLL motifs, one NLS, and one GRAS domain. The similarities of the protein amino acids with GAI, RGA, RGL... |
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| Keywords: | Gossypium barbadense L DELLA protein bioinformatics analysi |
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