| 特殊医学用途婴儿配方食品中乳铁蛋白的检测 |
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| 引用本文： | 管乐,代江华,江杏,章肇敏.特殊医学用途婴儿配方食品中乳铁蛋白的检测[J].乳业科学与技术,2018,41(4):21-24. |
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| 作者姓名： | 管乐 代江华 江杏 章肇敏 |
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| 作者单位： | 江西金薄金生态科技有限公司,江西 高安,330812 |
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| 摘 要： | 建立特殊医学用途婴儿配方食品中乳铁蛋白的高效液相色谱（high performance liquid chromatography，HPLC）检测方法。试样中的乳铁蛋白经磷酸盐缓冲液提取，肝素亲和柱净化后，用反相-HPLC分离，以0.1%三氟乙酸和乙腈作为流动相进行梯度洗脱，HPLC仪紫外检测器检测，外标法定量。结果表明：在乳铁蛋白质量浓度50～500 μg/mL范围内，检测结果呈现良好线性关系；该方法的检出限为5 mg/100 g，定量限为15 mg/100 g，平均回收率为93.9%；同一质量浓度乳铁
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| 关 键 词： | 特殊医学用途婴儿配方食品 乳铁蛋白 高效液相色谱 |
Detection of Lactoferrin in Formulas for Special Medical Purposes Intended for Infants |
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| GUAN Yue,DAI Jianghua,JIANG Xing,ZHANG Zhaomin.Detection of Lactoferrin in Formulas for Special Medical Purposes Intended for Infants[J].JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY,2018,41(4):21-24. |
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| Authors: | GUAN Yue DAI Jianghua JIANG Xing ZHANG Zhaomin |
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| Institution: | (Jiangxi Jinbojin Eco-technology Co. Ltd., Gao’an 330812, China) |
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| Abstract: | In this study, a high performance liquid chromatography (HPLC) method was developed for the determination of lactoferrin in formulas for special medical purposes intended for infants. Lactoferrin from samples was extracted with phosphate buffer. After being purified on a heparin affinity column, the analyte was separated by reversed-phase HPLC using gradient elution with 0.1% trifluoroacetic acid and acetonitrile mixture as the mobile phase, detected by a UV detector and quantitated by the external standard method. Results showed that good linearity was observed for lactoferrin in the concentration range of 50–500 μg/mL. The detection limit of the method was 5 mg/100 g, and the quantitation limit was 15 mg/100 g. The average recovery was 93.9%. The precision expressed as relative standard deviation (RSD) was 0.12%, and the stability RSD was 0.96%. Proficiency testing confirmed the reliability of the method. This method was convenient, fast, accurate, reliable and reproducible, and could effectively detect lactoferrin in formulas for special medical purposes intended for infants. |
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| Keywords: | formulas for special medical purposes intended for infants lactoferrin high performance liquid chromatography |
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