| Soleris微生物实时光电法与平板计数法测定生乳中菌落总数的比较研究 |
| |
| 引用本文： | 陈美莲,章慧,王仕英.Soleris微生物实时光电法与平板计数法测定生乳中菌落总数的比较研究[J].乳业科学与技术,2018,41(5):24-27. |
| |
| 作者姓名： | 陈美莲 章慧 王仕英 |
| |
| 作者单位： | 上海市农产品质量安全检测中心,农业部奶产品质量安全风险评估实验室(上海),上海 201708 |
| |
| 基金项目： | 国家农产品质量安全风险评估重大专项（GJFP201800804） |
| |
| 摘 要： | 建立Soleris微生物实时光电检测法检测生乳中菌落总数的快速测定方法，并将该方法与国标的平板计数法进行比较。结果表明：Soleris法检测生乳中菌落总数的标准曲线为y=－0.651 5x＋8.263 9（y为菌落总数（lg（CFU/mL）），x为胶体栓变色时间（h）），相关系数R2=－0.939 6，表明标准曲线相关性良好；重复性实验结果的相对标准偏差均小于5%；Soleris法与平板计数法测定结果的比较表明，2 种方法之间没有显著性差异（P＞0.05）。在本研究的实验条件下，Soleris法检测生乳中
|
| 关 键 词： | Soleris微生物实时光电检测 平板计数 生乳 菌落总数 |
Comparison of Soleris Real-Time Optoelectronic System with Aerobic Plate Count Method for Detection of Total Bacterial Count in Raw Milk |
| |
| CHEN Meilian,ZHANG Hui,WANG Shiying.Comparison of Soleris Real-Time Optoelectronic System with Aerobic Plate Count Method for Detection of Total Bacterial Count in Raw Milk[J].JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY,2018,41(5):24-27. |
| |
| Authors: | CHEN Meilian ZHANG Hui WANG Shiying |
| |
| Institution: | (Laboratory of Quality and Safety Risk Assessment for Dairy Product (Shanghai), Ministry of Agriculture Shanghai Agri-products Quality and Safety Testing Center, Shanghai 201708, China) |
| |
| Abstract: | A method using a Soleris real-time optoelectronic microbial detection system was developed for the rapid determination of the total bacterial count in raw milk and compared with the conventional aerobic plate count method (APC) in this paper. The standard curve equation of the Soleris method was established as follows: y = ?0.651 5x + 8.263 9, where y is total bacterial count (lg (CFU/mL)) and x is detection time (h) (R2 = ? 0.939 6) and it was verified to have good correlation. Repeatability tests showed relative standard deviation (RSD) values less than 5%. No statistically significant difference was observed comparing the two methods (P > 0.05). Under the experimental conditions in this study, it took 0.9–11.8 h to analyze one sample using the Soleris method, which was greatly shortened compared with that (48 h) using APC. Furthermore, the Soleris method was simple and rapid and allowed simultaneous detection of 128 samples. Accordingly, it could be considered as an efficient and stable method for the rapid determination of the total bacterial count in raw milk. |
| |
| Keywords: | Soleris real time optoelectronic microbial detection system aerobic plate count raw milk total bacterial count |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《乳业科学与技术》浏览原始摘要信息 |
| 点击此处可从《乳业科学与技术》下载免费的PDF全文 |
