| 鸭病毒性脑炎（暂定）病原分离与鉴定的初步研究 |
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| 引用本文： | 温立斌,张福军,王玉然,杨桂芝,马志华,解建平,杨浩.鸭病毒性脑炎（暂定）病原分离与鉴定的初步研究[J].中国兽医杂志,2001,37(2):3-4. |
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| 作者姓名： | 温立斌 张福军 王玉然 杨桂芝 马志华 解建平 杨浩 |
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| 作者单位： | 河北省动物检疫站, |
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| 摘 要： | 从以腹泻和腿麻痹为主要症状的康贝尔鸭肝、脾中分离到1株病毒。该病毒能适应于鸡胚、鸭胚，胚接种后不死亡，但有出血点。小鸭人工感染病毒后出现与自然病例相似的症状，并能回收到病毒。电镜观察病毒主要呈球形，有囊膜，大小约为30nm；病毒有3种结构蛋白，VP1（17KD）、VP2（41KD）和VP3（50KD），其中VP3为主要结构蛋白，病毒核酸为单链RNA。
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| 关 键 词： | 鸭 病毒 分离 鉴定 病毒性脑炎 |
| 文章编号： | 0529-6005(2001)02-0003-02 |
| 修稿时间： | 1999年12月28 |
Preliminary studies on
isolation and identification of a new virus causing encephalitis in ducks |
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| WEN Li-bin,ZHANG Fu-jun,WANG Yu-ran,YANG Gui-zhi,MA Zhi-hua,XIE Jian-ping,YANG Hao.Preliminary studies on
isolation and identification of a new virus causing encephalitis in ducks[J].Chinese Journal of Veterinary Medicine,2001,37(2):3-4. |
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| Authors: | WEN Li-bin ZHANG Fu-jun WANG Yu-ran YANG Gui-zhi MA Zhi-hua XIE Jian-ping YANG Hao |
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| Abstract: | one strain of virus was isolated from the spleens and livers of the ducks characterized mainly by diarrhea and paralysis on duck farms in Hebei province during 1995, The embryos inoculated with the virus didn't die. The ducklings inoculated with the virus showed symptoms similar to those of field cases,and the virus could be obtainted from the tissues of those died ducklings.By electron microscopy,the virus was spherical in shape and 30 nm in diameter approximately and in envelope.The SDS PAGE experiments revealed that the virus contained 3 structure polypeptides:VP 1(17KD),VP 2(41KD),VP 3(50KD).The VP 3 was a major structure protein,the test also showed the virus genome contained one single strnad RNA. |
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| Keywords: | duck virus isolation identification encephalitis |
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